Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus

被引：124

作者：

Honda, Y ^{[1
]}

Matsuyama, T ^{[1
]}

Irie, T ^{[1
]}

Watanabe, T ^{[1
]}

Kuwahara, M ^{[1
]}

机构：

[1] Kyoto Univ, Wood Res Inst, Kyoto 6110011, Japan

来源：

CURRENT GENETICS | 2000年 / 37卷 / 03期

关键词：

basidiomycete; transformation; white-rot fungi; carboxin; succinate : ubiquinone oxidoreductase;

D O I：

10.1007/s002940050521

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx(R), encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.

引用

页码：209 / 212

页数：4

共 12 条

[1] DNA-MEDIATED TRANSFORMATION OF THE BASIDIOMYCETE COPRINUS-CINEREUS
BINNINGER, DM
SKRZYNIA, C
PUKKILA, PJ
CASSELTON, LA
[J]. EMBO JOURNAL, 1987, 6 (04) : 835 - 840
[2] A SINGLE AMINO-ACID CHANGE IN THE IRON-SULFUR PROTEIN SUBUNIT OF SUCCINATE-DEHYDROGENASE CONFERS RESISTANCE TO CARBOXIN IN USTILAGO-MAYDIS
BROOMFIELD, PLE
HARGREAVES, JA
[J]. CURRENT GENETICS, 1992, 22 (02) : 117 - 121
[3] PRESENCE OF A BACTERIOPHAGE-P1 SEQUENCE IN TRANSFORMING PLASMIDS OF PLEUROTUS-OSTREATUS
HERZOG, RW
SINGH, NK
SCHMIDT, C
LEMKE, PA
[J]. CURRENT GENETICS, 1995, 27 (05) : 460 - 465
[4] Cloning and characterization of the gene encoding the iron-sulfur protein of succinate dehydrogenase from Pleurotus ostreatus
Irie, T
Honda, Y
Matsuyama, T
Watanabe, T
Kuwahara, M
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1998, 1396 (01): : 27 - 31
[5] Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungus Pleurotus ostreatus
Irie, T
Honda, Y
Matsuyama, T
Watanabe, T
Kuwahara, M
[J]. JOURNAL OF WOOD SCIENCE, 1998, 44 (06) : 491 - 494
[6] ISOLATION, CHARACTERIZATION AND SEQUENCE OF A GENE CONFERRING RESISTANCE TO THE SYSTEMIC FUNGICIDE CARBOXIN FROM THE MAIZE SMUT PATHOGEN, USTILAGO-MAYDIS
KEON, JPR
WHITE, GA
HARGREAVES, JA
[J]. CURRENT GENETICS, 1991, 19 (06) : 475 - 481
[7] A FAMILY OF USTILAGO-MAYDIS EXPRESSION VECTORS - NEW SELECTABLE MARKERS AND PROMOTERS
KINAL, H
PARK, CM
BRUENN, JA
[J]. GENE, 1993, 127 (01) : 151 - 152
[8] KOFUJITA H, 1991, MOKUZAI GAKKAISHI, V37, P555
[9] RECOVERY OF RECOMBINANT PLASMIDS FROM PLEUROTUS-OSTREATUS TRANSFORMANTS
PENG, M
SINGH, NK
LEMKE, PA
[J]. CURRENT GENETICS, 1992, 22 (01) : 53 - 59
[10] PENG M, 1993, APPL MICROBIOL BIOT, V40, P101

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