Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungus Pleurotus ostreatus

We have isolated a structural gene of sdi1, which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase (EC 1.3.99.1), from a white-rot basidiomycete, Pleurotus ostreatus. Here we report isolation of the promoter region of sdi1 and an allelic sequence encoding the second-type cDNA fragment isolated in the former experiments. The nucleotide sequence analysis of the promoter region revealed the existence of putative CAAT and TATA boxes, which permits us to develop an expression system in this species. The Southern blot analysis and the restriction fragment length polymorphism assay using monokaryotic strains demonstrated that no family genes to sdi1 exist in the haploid genome of P. ostreatus. Moreover, a genetic analysis to detect a linkage between the sdi1 genotypes and flutolanil resistance in the mutant P. ostreatus strains was also developed.