Cloning and characterization of the gene encoding the iron-sulfur protein of succinate dehydrogenase from Pleurotus ostreatus

被引:9
作者
Irie, T [1 ]
Honda, Y [1 ]
Matsuyama, T [1 ]
Watanabe, T [1 ]
Kuwahara, M [1 ]
机构
[1] Kyoto Univ, Wood Res Inst, Kyoto 611, Japan
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1396卷 / 01期
关键词
succinate : ubiquinone oxidoreductase; mitochondrial targeting peptide; (basidiomycete); (white-rot fungi);
D O I
10.1016/S0167-4781(97)00203-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genomic and cDNA fragments encoding the iron-sulfur protein (Ip) subunit of succinate dehydrogenase (EC 1.3.99.1) have been cloned from the edible basidiomycetous fungus, Pleurotus ostreatus. The gene is interrupted by five introns and is predicted to encode a polypeptide of 268 amino acid residues. Sequence comparison with the Ip subunit from other species identified three conserved cysteine-rich clusters. One of these contains a critical histidine residue implicated in carboxin sensitivity in the heterobasidiomycete Ustilago maydis. (C) 1998 Elsevier Science B.V.
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页码:27 / 31
页数:5
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