Simultaneous determination of nicotinic acid and its metabolites in rat urine by micellar electrokinetic chromatography with photodiode away detection

被引：17

作者：

Iwaki, M ^{[1
]}

Murakami, E ^{[1
]}

Kikuchi, M ^{[1
]}

Wada, A ^{[1
]}

Ogiso, T ^{[1
]}

Oda, Y ^{[1
]}

Kubo, K ^{[1
]}

Kakehi, K ^{[1
]}

机构：

[1] Kinki Univ, Fac Pharmaceut Sci, Higashiosaka, Osaka 577, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 716卷 / 1-2期

关键词：

nicotinic acid; nicotinamide;

D O I：

10.1016/S0378-4347(98)00327-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nicotinic acid, nicotinamide and their possible metabolites were successfully separated within 17 min by micellar electrokinetic chromatography using 50 mM berate buffer (pH 9.0) containing 150 mM sodium dodecyl sulfate as the running buffer. Calibration curves for all compounds showed good linearity in a range of 5 mu g/ml and 250 mu g/ml with good correlation. The present method did not require any clean-up procedures and made it possible to determine all metabolites without interference on a photodiode array detector. Urine samples collected from Wistar male rats were analyzed after high-dose oral or intravenous administration of nicotinic acid or nicotinamide. Metabolic pathways of nicotinic acid in male Wistar rats are also discussed. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：335 / 342

页数：8

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