Coordinated circRNA Biogenesis and Function with NF90/NF110 in Viral Infection

被引：782

作者：

Li, Xiang ^{[1
,2
]}

Liu, Chu-Xiao ^{[1
,2
]}

Xue, Wei ^{[3
]}

Zhang, Yang ^{[1
,2
]}

Jiang, Shan ^{[1
,2
]}

Yin, Qing-Fei ^{[1
,2
]}

Wei, Jia ^{[3
]}

Yao, Run-Wen ^{[1
,2
]}

Yang, Li ^{[3
,4
]}

Chen, Ling-Ling ^{[1
,2
,4
]}

机构：

[1] Univ Chinese Acad Sci, Chinese Acad Sci, State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China

[2] Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Key Lab Mol Androl, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China

[3] Univ Chinese Acad Sci, Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci,Key Lab Computat Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China

[4] ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China

来源：

MOLECULAR CELL | 2017年 / 67卷 / 02期

关键词：

RNA-PROTEIN INTERACTIONS; MESSENGER-RNA; CIRCULAR RNAS; NONCODING RNAS; BINDING; NF90; PHOSPHORYLATION; MECHANISM; TRANSLATION; REVEALS;

D O I：

10.1016/j.molcel.2017.05.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Circular RNAs (circRNAs) generated via back-splicing are enhanced by flanking complementary sequences. Expression levels of circRNAs vary under different conditions, suggesting participation of protein factors in their biogenesis. Using genome-wide siRNA screening that targets all human unique genes and an efficient circRNA expression reporter, we identify double-stranded RNA-binding domain containing immune factors NF90/NF110 as key regulators in circRNA biogenesis. NF90/NF110 promote circRNA production in the nucleus by associating with intronic RNA pairs juxtaposing the circRNA-forming exon(s); they also interact with mature circRNAs in the cytoplasm. Upon viral infection, circRNA expression is decreased, in part owing to the nuclear export of NF90/NF110 to the cytoplasm. Meanwhile, NF90/NF110 released from circRNP complexes bind to viral mRNAs as part of their functions in antiviral immune response. Our results therefore implicate a coordinated regulation of circRNA biogenesis and function by NF90/NF110 in viral infection.

引用

页码：214 / +

页数：21

共 53 条

[1]

circRNA Biogenesis Competes with Pre-mRNA Splicing [J].

Ashwal-Fluss, Reut ;

Meyer, Markus ;

Pamudurti, Nagarjuna Reddy ;

Ivanov, Andranik ;

Bartok, Osnat ;

Hanan, Mor ;

Evantal, Naveh ;

Memczak, Sebastian ;

Rajewsky, Nikolaus ;

Kadener, Sebastian .

MOLECULAR CELL, 2014, 56 (01) :55-66

[2]

Bailey T L, 1994, Proc Int Conf Intell Syst Mol Biol, V2, P28

[3]

UniProt: the universal protein knowledgebase [J].

Bateman, Alex ;

Martin, Maria Jesus ;

O'Donovan, Claire ;

Magrane, Michele ;

Alpi, Emanuele ;

Antunes, Ricardo ;

Bely, Benoit ;

Bingley, Mark ;

Bonilla, Carlos ;

Britto, Ramona ;

Bursteinas, Borisas ;

Bye-A-Jee, Hema ;

Cowley, Andrew ;

Da Silva, Alan ;

De Giorgi, Maurizio ;

Dogan, Tunca ;

Fazzini, Francesco ;

Castro, Leyla Garcia ;

Figueira, Luis ;

Garmiri, Penelope ;

Georghiou, George ;

Gonzalez, Daniel ;

Hatton-Ellis, Emma ;

Li, Weizhong ;

Liu, Wudong ;

Lopez, Rodrigo ;

Luo, Jie ;

Lussi, Yvonne ;

MacDougall, Alistair ;

Nightingale, Andrew ;

Palka, Barbara ;

Pichler, Klemens ;

Poggioli, Diego ;

Pundir, Sangya ;

Pureza, Luis ;

Qi, Guoying ;

Rosanoff, Steven ;

Saidi, Rabie ;

Sawford, Tony ;

Shypitsyna, Aleksandra ;

Speretta, Elena ;

Turner, Edward ;

Tyagi, Nidhi ;

Volynkin, Vladimir ;

Wardell, Tony ;

Warner, Kate ;

Watkins, Xavier ;

Zaru, Rossana ;

Zellner, Hermann ;

Xenarios, Ioannis .

NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D158-D169

[4]

A guided tour into subcellular colocalization analysis in light microscopy [J].

Bolte, S. ;

Cordelieres, F. P. .

JOURNAL OF MICROSCOPY, 2006, 224 (213-232) :213-232

[5]

Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins [J].

Castello, Alfredo ;

Fischer, Bernd ;

Eichelbaum, Katrin ;

Horos, Rastislav ;

Beckmann, Benedikt M. ;

Strein, Claudia ;

Davey, Norman E. ;

Humphreys, David T. ;

Preiss, Thomas ;

Steinmetz, Lars M. ;

Krijgsveld, Jeroen ;

Hentze, Matthias W. .

CELL, 2012, 149 (06) :1393-1406

[6]

The biogenesis and emerging roles of circular RNAs [J].

Chen, Ling-Ling .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2016, 17 (04) :205-211

[7]

ADAR1 is required for differentiation and neural induction by regulating microRNA processing in a catalytically independent manner [J].

Chen, Tian ;

Xiang, Jian-Feng ;

Zhu, Shanshan ;

Chen, Siye ;

Yin, Qing-Fei ;

Zhang, Xiao-Ou ;

Zhang, Jun ;

Feng, Hua ;

Dong, Rui ;

Li, Xue-Jun ;

Yang, Li ;

Chen, Ling-Ling .

CELL RESEARCH, 2015, 25 (04) :459-476

[8]

Choe J, 2005, SCIENCE, V309, P581, DOI 10.1126/science.1115253

[9]

The RNA Binding Protein Quaking Regulates Formation of circRNAs [J].

Conn, Simon J. ;

Pillman, Katherine A. ;

Toubia, John ;

Conn, Vanessa M. ;

Salmanidis, Marika ;

Phillips, Caroline A. ;

Roslan, Suraya ;

Schreiber, Andreas W. ;

Gregory, Philip A. ;

Goodall, Gregory J. .

CELL, 2015, 160 (06) :1125-1134

[10]

Dong R, 2016, RNA BIOL, P1

← 1 2 3 4 5 6 →