Peptide synthesis in organic media with subtilisin 72 immobilized on poly(vinyl alcohol)-cryogel carrier

被引：42

作者：

Bacheva, AV

Plieva, FM

Lysogorskaya, EN

Filippova, IY

Lozinsky, VI

机构：

[1] Moscow MV Lomonosov State Univ, Fac Chem, Dept Chem Nat Cpds, Moscow 119899, Russia

[2] Russian Acad Sci, AN Nesmeyanov Organoelement Cpds Inst, Moscow 117813, Russia

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2001年 / 11卷 / 08期

基金：

俄罗斯基础研究基金会;

关键词：

D O I：

10.1016/S0960-894X(01)00113-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Serine proteinase subtilisin 72 was covalently attached to the beads of poly(vinyl alcohol)-cryogel, a macroporous hydrogel prepared by the freeze-thaw technique. The immobilized enzyme was examined as a catalyst in the synthesis of protected peptides Z-Ala-Ala-Xaa-Phe-pNA (Xaa = Leu. Glu, Lys) in acetonitrile/dimethylformamide mixtures. Immobilized subtilisin catalyzed with high yield the formation of peptide bonds between Phe-pNA and acyl donors including those with free carboxylic group and non-protected C-terminal basic and acidic amino acid residues. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：1005 / 1008

页数：4

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