MLVA and SNP analysis identified a unique genetic cluster in Bulgarian Bacillus anthracis strains

被引：18

作者：

Antwerpen, M. ^{[1
]}

Ilin, D. ^{[2
]}

Georgieva, E. ^{[2
]}

Meyer, H. ^{[1
]}

Savov, E. ^{[2
]}

Frangoulidis, D. ^{[1
]}

机构：

[1] Bundeswehr Inst Microbiol, D-80937 Munich, Germany

[2] Mil Med Acad, Dept Clin Microbiol, Sofia, Bulgaria

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2011年 / 30卷 / 07期

关键词：

INHALATIONAL ANTHRAX; DIVERSITY; KAMEIDO;

D O I：

10.1007/s10096-011-1177-2

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A collection of 40 Bacillus anthracis strains mostly isolated from soil in Bulgaria between 1960 and 1980 were investigated. All strains were proven to be B. anthracis by culture and amplification of a B. anthracis-specific chromosomal marker. PCR demonstrated that in nine strains both virulence plasmids (pX01+/pX02+) and in four strains only one plasmid (pX02+) were present, whereas the majority of strains (n = 27) lacked both plasmids (pX01-/pX02-). Multi-locus-variable number of tandem repeat-analysis (MLVA) using 15 markers differentiated three genotypes. Comparison with typing data of more than 1,000 different B. anthracis strains revealed that Bulgarian genotypes affiliated with the A1.a cluster and form their own unique cluster different from clusters containing strains isolated in geographical proximity, e.g., Turkey, Georgia, Hungary, Albania or Italy. In addition, a new allele of one marker (vrrC2) was identified. Canonical single nucleotide polymorphisms analysis allocated 31 Bulgarian strains into the A.Br.008/009 and nine strains into the A.Br.WNA group, which is the first description of B. anthracis strains of the A.Br.WNA group on the Eurasian continent.

引用

页码：923 / 930

页数：8

共 25 条

[11] Pre-Columbian Origins for North American Anthrax [J].

Kenefic, Leo J. ;

Pearson, Talima ;

Okinaka, Richard T. ;

Schupp, James M. ;

Wagner, David M. ;

Ravel, Jacques ;

Hoffmaster, Alex R. ;

Trim, Carla P. ;

Chung, Wai-Kwan ;

Beaudry, Jodi A. ;

Foster, Jeffrey T. ;

Mead, James I. ;

Keim, Paul .

PLOS ONE, 2009, 4 (03)

[12] Genome-Wide Single Nucleotide Polymorphism Typing Method for Identification of Bacillus anthracis Species and Strains among B. cereus Group Species [J].

Kuroda, Makoto ;

Serizawa, Masakuni ;

Okutani, Akiko ;

Sekizuka, Tsuyoshi ;

Banno, Satomi ;

Inoue, Satoshi .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (08) :2821-2829

[13]

Le Fleche P, 2001, BMC Microbiol, V1, P2

[14] Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variable-number tandem repeats analysis [J].

Lista, Florigio ;

Faggioni, Giovanni ;

Valjevac, Samina ;

Ciammaruconi, Andrea ;

Vaissaire, Josee ;

le Doujet, Claudine ;

Gorge, Olivier ;

De Santis, Riccardo ;

Carattoli, Alessandra ;

Ciervo, Alessandra ;

Fasanella, Antonio ;

Orsini, Francesco ;

D'Amelio, Raffaele ;

Pourcel, Christine ;

Cassone, Antonio ;

Vergnaud, Gilles .

BMC MICROBIOLOGY, 2006, 6 (1)

[15] Effects of long-term storage on plasmid stability in Bacillus anthracis [J].

Marston, CK ;

Hoffmaster, AR ;

Wilson, KE ;

Bragg, SL ;

Plikaytis, B ;

Brachman, P ;

Johnson, S ;

Kaufmann, AF ;

Popovic, T .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :7778-7780

[16] Diversity of Bacillus anthracis strains in Georgia and of vaccine strains from the former Soviet Union [J].

Merabishvili, Maya ;

Natidze, Merab ;

Rigvava, Sergo ;

Brusetti, Lorenzo ;

Raddadi, Noura ;

Borin, Sara ;

Chanishvili, Nina ;

Tediashvili, Marina ;

Sharp, Richard ;

Barbeschi, Maurizio ;

Visca, Paolo ;

Daffonchio, Daniele .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (08) :5631-5636

[17] Phylogenetic discovery bias in Bacillus anthracis using single-nucleotide polymorphisms from whole-genome sequencing [J].

Pearson, T ;

Busch, JD ;

Ravel, J ;

Read, TD ;

Rhoton, SD ;

U'Ren, JM ;

Simonson, TS ;

Kachur, SM ;

Leadem, RR ;

Cardon, ML ;

Van Ert, MN ;

Huynh, LY ;

Fraser, CM ;

Keim, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (37) :13536-13541

[18] Molecular epidemiology of Bacillus anthracis:: Determining the correct origin [J].

Pilo, Paola ;

Perreten, Vincent ;

Frey, Joachim .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (09) :2928-2931

[19] Bacillus anthracis in China and its relationship to worldwide lineages [J].

Simonson, Tatum S. ;

Okinaka, Richard T. ;

Wang, Bingxiang ;

Easterday, W. Ryan ;

Huynh, Lynn ;

U'Ren, Jana M. ;

Dukerich, Meghan ;

Zanecki, Shaylan R. ;

Kenefic, Leo J. ;

Beaudry, Jodi ;

Schupp, James M. ;

Pearson, Talima ;

Wagner, David M. ;

Hoffmaster, Alex ;

Ravel, Jacques ;

Keim, Paul .

BMC MICROBIOLOGY, 2009, 9

[20] Bacillus anthracis incident, Kameido, Tokyo, 1993 [J].

Takahashi, H ;

Keim, P ;

Kaufmann, AF ;

Keys, C ;

Smith, KL ;

Taniguchi, K ;

Inouye, S ;

Kurata, T .

EMERGING INFECTIOUS DISEASES, 2004, 10 (01) :117-120

← 1 2 3 →