Structural and Thermodynamic Comparison of the Catalytic Domain of AMSH and AMSH-LP: Nearly Identical Fold but Different Stability

被引:45
作者
Davies, Christopher W. [1 ]
Paul, Lake N. [2 ]
Kim, Myung-Ii [1 ]
Das, Chittaranjan [1 ]
机构
[1] Purdue Univ, Dept Chem, Brown Lab Chem, W Lafayette, IN 47907 USA
[2] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA
基金
美国国家卫生研究院;
关键词
endosomal deubiquitinase; Lys63-linked polyubiquitin-chain-specific deubiquitinase; JAMM domain deubiquitinase; metalloprotease; conformational plasticity; SPLICING FACTOR PRP8; DEUBIQUITINATING ENZYMES; RETINITIS-PIGMENTOSA; JAB1/MPN DOMAIN; ESCRT; PROTEIN; CYTOKINESIS; ENDOSOMES; MECHANISM; ULTRACENTRIFUGATION;
D O I
10.1016/j.jmb.2011.08.029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
AMSH plays a critical role in the ESCRT (endosomal sorting complexes required for transport) machinery, which facilitates the down-regulation and degradation of cell-surface receptors. It displays a high level of specificity toward cleavage of Lys63-linked polyubiquitin chains, the structural basis of which has been understood recently through the crystal structure of a highly related, but ESCRT-independent, protein AMSH-LP (AMSH-like protein). We have determined the X-ray structure of two constructs representing the catalytic domain of AMSH: AMSH244, the JAMM (JAB1/MPN/MOV34)-domain-containing polypeptide segment from residues 244 to 424, and AMSH219(E280A), an active-site mutant, Glu280 to Ala, of the segment from 219 to 424. In addition to confirming the expected zinc coordination in the protein, the structures reveal that the catalytic domains of AMSH and AMSH-LP are nearly identical; however, guanidine-hydrochloride-induced unfolding studies show that the catalytic domain of AMSH is thermodynamically less stable than that of AMSH-LP, indicating that the former is perhaps structurally more plastic. Much to our surprise, in the AMSH219(E280A) structure, the catalytic zinc was still held in place, by the compensatory effect of an aspartate from a nearby loop moving into a position where it could coordinate with the zinc, once again suggesting the plasticity of AMSH. Additionally, a model of AMSH244 bound to Lys63-linked diubiquitin reveals a type of interface for the distal ubiquitin significantly different from that seen in AMSH-LP. Altogether, we believe that our data provide important insight into the structural difference between the two proteins that may translate into the difference in their biological function. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:416 / 429
页数:14
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