Synthetically modified DNAs as substrates for polymerases

被引：73

作者：

Kool, ET ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2000年 / 4卷 / 06期

关键词：

D O I：

10.1016/S1367-5931(00)00141-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA polymerase enzymes process their natural substrates with very high specificity. Yet recent experiments have shown that these enzymes can also process DNA in which the backbone or bases are modified to a surprising degree. Such experiments have important implications in understanding the mechanisms of DNA replication, and suggest important biotechnological uses as well.

引用

页码：602 / 608

页数：7

共 55 条

[1] PCR-based development of DNA substrates containing modified bases: An efficient system for investigating the role of the exocyclic groups in chemical and structural recognition by minor groove binding drugs and proteins
Bailly, C
Payet, D
Travers, AA
Waring, MJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) : 13623 - 13628
[2] RIBOSOME-MEDIATED INCORPORATION OF A NONSTANDARD AMINO-ACID INTO A PEPTIDE THROUGH EXPANSION OF THE GENETIC-CODE
BAIN, JD
SWITZER, C
CHAMBERLIN, AR
BENNER, SA
[J]. NATURE, 1992, 356 (6369) : 537 - 539
[3] Quantitative analysis of receptors for adenosine nucleotides obtained via in vitro selection from a library incorporating a cationic nucleotide analog
Battersby, TR
Ang, DN
Burgstaller, P
Jurczyk, SC
Bowser, MT
Buchanan, DD
Kennedy, RT
Benner, SA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (42) : 9781 - 9789
[4] A minor groove binding track in reverse transcriptase
Bebenek, K
Beard, WA
Darden, TA
Li, LP
Prasad, R
Luxon, BA
Gorenstein, DG
Wilson, SH
Kunkel, TA
[J]. NATURE STRUCTURAL BIOLOGY, 1997, 4 (03) : 194 - 197
[5] Redesigning nucleic acids
Benner, SA
Battersby, TR
Eschgfaller, B
Hutter, D
Kodra, JT
Lutz, S
Arslan, T
Baschlin, DK
Blattler, M
Egli, M
Hammer, C
Held, HA
Horlacher, J
Huang, Z
Hyrup, B
Jenny, TF
Jurczyk, SC
Konig, M
von Krosigk, U
Lutz, MJ
MacPherson, LJ
Moroney, SE
Muller, E
Nambiar, KP
Piccirilli, JA
Switzer, CY
Vogel, JJ
Richert, C
Roughton, AL
Schmidt, J
Schneider, KC
Stackhouse, J
[J]. PURE AND APPLIED CHEMISTRY, 1998, 70 (02) : 263 - 266
[6] Dye structure affects Taq DNA polymerase terminator selectivity
Brandis, JW
[J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (08) : 1912 - 1918
[7] UNAMBIGUOUS DETERMINATION OF THE STEREOCHEMISTRY OF NUCLEOTIDYL TRANSFER CATALYZED BY DNA-POLYMERASE-I FROM ESCHERICHIA-COLI
BRODY, RS
FREY, PA
[J]. BIOCHEMISTRY, 1981, 20 (05) : 1245 - 1252
[8] BURGERS PMJ, 1979, J BIOL CHEM, V254, P6889
[9] ROLLING-CIRCLE RNA-SYNTHESIS - CIRCULAR OLIGONUCLEOTIDES AS EFFICIENT SUBSTRATES FOR T7 RNA-POLYMERASE
DAUBENDIEK, SL
RYAN, K
KOOL, ET
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (29) : 7818 - 7819
[10] Generation of catalytic RNAs by rolling transcription of synthetic DNA nanocircles
Daubendiek, SL
Kool, ET
[J]. NATURE BIOTECHNOLOGY, 1997, 15 (03) : 273 - 277

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