Is RE1/NRSE a common cis-regulatory sequence for ChAT and VAChT genes?

被引：17

作者：

De Gois, S ^{[1
]}

Houhou, L ^{[1
]}

Oda, Y ^{[1
]}

Corbex, M ^{[1
]}

Pajak, F ^{[1
]}

Thévenot, E ^{[1
]}

Vodjdani, G ^{[1
]}

Mallet, J ^{[1
]}

Berrard, S ^{[1
]}

机构：

[1] Hop La Pitie Salpetriere, CNRS, UMRC 9935, Lab Genet Mol Neurotransmiss & Proc, F-75013 Paris, France

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 47期

关键词：

D O I：

10.1074/jbc.M006895200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Choline acetyltransferase (ChAT), the biosynthetic enzyme of acetylcholine, and the vesicular acetylcholine transporter (VAChT) are both required for cholinergic neurotransmission. These proteins are encoded by two embedded genes, the VAChT gene lying within the first intron of the ChAT gene. In the nervous system, both ChAT and VAChT are synthesized only in cholinergic neurons, and it is therefore likely that the cell type-specific expression of their genes is coordinately regulated. It has been suggested that a. 2336-base pair genomic region upstream from the ChAT and VAChT coding sequences drives ChAT gene expression in cholinergic structures. We investigated whether this region also regulates VAChT gene transcription. Transfection assays showed that this region strongly represses the activity of the native VAChT promoters in non-neuronal cells, but has no major effect in neuronal cells whether or not they express the endogenous ChAT and VAChT genes. The silencer activity of this region is mediated solely by a repressor element 1 or neuron-restrictive silencer element (RE1/NRSE). Moreover, several proteins, including RE1-silencing transcription factor or neuron-restrictive silencer factor, are recruited by this regulatory sequence. These data suggest that this upstream region and RE1/NRSE co-regulate the expression of the ChAT and VAChT genes.

引用

页码：36683 / 36690

页数：8

共 55 条

[11] REST - A MAMMALIAN SILENCER PROTEIN THAT RESTRICTS SODIUM-CHANNEL GENE-EXPRESSION TO NEURONS [J].