Labeling tetracysteine-tagged proteins with a SplAsH of color: A modular approach to bis-arsenical fluorophores

被引:29
作者
Bhunia, Anjan K. [1 ]
Miller, Stephen C. [1 ]
机构
[1] Univ Massachusetts, Sch Med, Worcester, MA 01605 USA
关键词
D O I
10.1002/cbic.200700192
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
(Chemical Equation Presented) Everything nicely tied up. We have synthesized a non-fluorescent bis-arsenical targeting moiety that can be tethered to a wide variety of different fluorescent payloads. This strategy greatly broadens the scope of dyes that can be used to label tetracysteine-tagged proteins. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:1642 / 1645
页数:4
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