Influence of epiphytic micro-organisms on leaf wettability: wetting of the upper leaf surface of Juglans regia and of model surfaces in relation to colonization by micro-organisms

被引:26
作者
Knoll, D [1 ]
Schreiber, L [1 ]
机构
[1] Univ Wurzburg, Lehrstuhl Bot 2, Julius von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany
关键词
epiphytic micro-organisms; Fuglans regia L; leaf surface; leaf wettability; plant cuticle;
D O I
10.1046/j.1469-8137.1998.00269.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Wetting of the upper leaf surface of Juglans regia L. and of model surfaces colonized by epiphytic micro-organisms was investigated by measuring contact angles of aqueous solutions buffered at different pH values. During June to October 1995, contact angles of aqueous solutions on the leaf surface of J. regia decreased by angles ranging from 12 degrees (low pH values) to 25 degrees at high pH values. At the end of this vegetation period, wetting was strongly dependent on pH showing significantly lower contact angles with alkaline solutions (pH 9.0) than with acidic solutions (pH 3.0). Contact angle titration measured angles on the leaf surface as a function of the pH of buffered aqueous solutions, covering a pH range from 3.0 to 11.0. Titration curves revealed inflection points around 7.5, indicating the existence of ionizable carboxylic groups at the interface of the phylloplane. Altered leaf-surface wetting properties observed on the intact leaf surface could be simulated in model experiments by measuring contact angles on artificial surfaces colonized by Pseudomonas fluorescens and by epiphytic micro-organisms isolated from the phylloplane of J. regia. Strong evidence is provided that interfacial carboxylic groups derive from epiphytic micro-organisms present on the phylloplane. Results suggest that the age-dependent increase in, and pH dependence of, wetting as leaves mature are related to the presence of epiphytic micro-organisms on the phylloplane. Ecological consequences of increased leaf-surface wetting, concerning the structure of the leaf surface as a microhabitat for epiphytic micro-organisms, are discussed.
引用
收藏
页码:271 / 282
页数:12
相关论文
共 50 条
[41]   DETERMINATION OF FOLIAR UPTAKE OF CHEMICALS - INFLUENCE OF LEAF SURFACE MICROFLORA [J].
SCHREIBER, L ;
SCHONHERR, J .
PLANT CELL AND ENVIRONMENT, 1993, 16 (06) :743-748
[42]   Wetting of the upper needle surface of Abies grandis: Influence of pH, wax chemistry and epiphyllic microflora on contact angles [J].
Schreiber, L .
PLANT CELL AND ENVIRONMENT, 1996, 19 (04) :455-463
[43]  
Sentandreu R., 1994, MYCOTA, P111, DOI DOI 10.1007/978-3-662-11908-2_6
[44]   DIVERSITY OF CUTINASES FROM PLANT PATHOGENIC FUNGI - EVIDENCE FOR A RELATIONSHIP BETWEEN ENZYME PROPERTIES AND TISSUE-SPECIFICITY [J].
TRAIL, F ;
KOLLER, W .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1990, 36 (06) :495-508
[45]   LEACHING OF SUBSTANCES FROM PLANTS [J].
TUKEY, HB .
ANNUAL REVIEW OF PLANT PHYSIOLOGY, 1970, 21 :305-+
[46]   A REVIEW OF THE RESPONSE OF EPICUTICULAR WAX OF CONIFER NEEDLES TO AIR-POLLUTION [J].
TURUNEN, M ;
HUTTUNEN, S .
JOURNAL OF ENVIRONMENTAL QUALITY, 1990, 19 (01) :35-45
[47]   PARTICIPATION OF ORYZA-SATIVA LEAF WAX IN APPRESSORIUM FORMATION BY PYRICULARIA-ORYZAE [J].
UCHIYAMA, T ;
OKUYAMA, K .
PHYTOCHEMISTRY, 1990, 29 (01) :91-92
[48]  
Wicken A. J., 1985, BACTERIAL ADHESION M, P45
[49]   ADHESION OF COLLETOTRICHUM-LINDEMUTHIANUM SPORES TO PHASEOLUS-VULGARIS HYPOCOTYLS AND TO POLYSTYRENE [J].
YOUNG, DH ;
KAUSS, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (04) :616-619
[50]   Bacterial spot of peach as influenced by water congestion, leaf wetness duration, and temperature [J].
Zehr, EI ;
Shepard, DP ;
Bridges, WC .
PLANT DISEASE, 1996, 80 (03) :339-341