Expression of Aspergillus aculeatus no. F-50 cellobiohydrolase I (cbhI) and β-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae

被引：46

作者：

Takada, G ^{[1
]}

Kawaguchi, T ^{[1
]}

Sumitani, J ^{[1
]}

Arai, M ^{[1
]}

机构：

[1] Univ Osaka Prefecture, Coll Agr, Dept Appl Biol Chem, Sakai, Osaka 5998531, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 08期

关键词：

Aspergillus aculeatus; cellobiohydrolase; beta-glucosidase; synergism;

D O I：

10.1271/bbb.62.1615

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cellobiohydrolase I (cbhI) and a beta-glucosidase 1 (bgl1) gene of Aspergillus aculeatus were expressed in Saccharomyces cerevisiae. The transformed cells secreted the enzymes efficiently in an active form. The recombinant CBHI gave two bands of different molecular mass (110 and 90 kDa) and the recombinant BGL1 gave one band (180 kDa) by SDS-PAGE. The recombinant CBHI and BGL1 had the same enzymatical properties as the native enzyme except for the specific activity toward cellulosic substrates. By the combination of three different types of cellulases, FI-CMCase, CBHI, and BGL1, we could hydrolyze Avicel up to 59% under our experimental conditions.

引用

页码：1615 / 1618

页数：4

共 14 条

[1] CHARACTERIZATION OF CELLULOLYTIC ENZYME COMPONENTS FROM ASPERGILLUS-TERREUS AND ITS MUTANT [J].

ARAUJO, A ;

DSOUZA, J .

JOURNAL OF FERMENTATION TECHNOLOGY, 1986, 64 (05) :463-467

[2]

CHERNOGLAZOV V M, 1985, Biokhimiya, V50, P1108

[3] Cloning and sequencing of the cDNA encoding beta-glucosidase 1 from Aspergillus aculeatus [J].

Kawaguchi, T ;

Enoki, T ;

Tsurumaki, S ;

Sumitani, J ;

Ueda, M ;

Ooi, T ;

Arai, M .

GENE, 1996, 173 (02) :287-288

[4] CALORIMETRIC VERSUS GROWTH MICROBIAL ANALYSIS OF CELLULASE ENZYMES ACTING ON CELLULOSE [J].

LOVRIEN, RE ;

WILLIAMS, KK ;

FERREY, ML ;

AMMEND, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (12) :2935-2941

[5] SECRETIVE EXPRESSION OF THE ASPERGILLUS-ACULEATUS CELLULASE (FI-CMCASE) BY SACCHAROMYCES-CEREVISIAE [J].

MINAMIGUCHI, K ;

OOI, T ;

KAWAGUCHI, T ;

OKADA, H ;

MURAO, S ;

ARAI, M .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, 79 (04) :363-366

[6]

MURAO S, 1979, J FERMENT TECHNOL, V57, P151

[7] CLONING AND SEQUENCE-ANALYSIS OF A CDNA FOR CELLULASE (FI-CMCASE) FROM ASPERGILLUS-ACULEATUS [J].

OOI, T ;

SHINMYO, A ;

OKADA, H ;

HARA, S ;

IKENAKA, T ;

MURAO, S ;

ARAI, M .

CURRENT GENETICS, 1990, 18 (03) :217-222

[8] COMPLETE NUCLEOTIDE-SEQUENCE OF A GENE CODING FOR ASPERGILLUS-ACULEATUS CELLULASE (FI-CMCASE) [J].

OOI, T ;

SHINMYO, A ;

OKADA, H ;

MURAO, S ;

KAWAGUCHI, T ;

ARAI, M .

NUCLEIC ACIDS RESEARCH, 1990, 18 (19) :5884-5884

[9] EXPRESSION OF THE CELLULASE (FI-CMCASE) GENE OF ASPERGILLUS-ACULEATUS IN SACCHAROMYCES-CEREVISIAE [J].

OOI, T ;

MINAMIGUCHI, K ;

KAWAGUCHI, T ;

OKADA, H ;

MURAO, S ;

ARAI, M .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (05) :954-956

[10] SYNERGISM BETWEEN ENZYMES OF SCLEROTIUM-ROLFSII INVOLVED IN THE SOLUBILIZATION OF CRYSTALLINE CELLULOSE [J].

SADANA, JC ;

PATIL, RV .

CARBOHYDRATE RESEARCH, 1985, 140 (01) :111-120

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