Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue

被引：20

作者：

Bonnard, E ^{[1
]}

Burlet-Schiltz, O ^{[1
]}

Monsarrat, B ^{[1
]}

Girard, JP ^{[1
]}

Zajac, JM ^{[1
]}

机构：

[1] Inst Pharmacol & Biol Struct, F-31077 Toulouse, France

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2003年 / 270卷 / 20期

关键词：

neuropeptide FF; electrospray tandem mass spectrometry; precursor processing; neuroblastoma;

D O I：

10.1046/j.1432-1033.2003.03816.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptides which should be generated from the neuropeptide FF (NPFF) precursor were identified in a neuronal (human neuroblastoma SH-SY5Y) cell line and in COS-7 cells after transient transfection of the human proNPFF(A) cDNA and were compared with those detected in the mouse spinal cord. After reverse-phase high performance liquid chromatography of soluble material, NPFF-related peptides were immunodetected with antisera raised against NPFF and identified by using on-line capillary liquid chromatography/ nanospray ion trap tandem mass spectrometry. Neuronal and non-neuronal cells generated different peptides from the same precursor. In addition to NPFF, SQA-NPFF (Ser-Gln-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide) and NPAF were identified in the human neuroblastoma while only NPFF was clearly identified in COS-7 cells. In mouse, in addition to previously detected NPFF and NPSF, SPA-NPFF (Ser-Pro-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide), the homologous peptide of SQA-NPFF, were characterized. These data on intracellular processing of proNeuropeptide FFA are discussed in regard to the known enzymatic processing mechanisms.

引用

页码：4187 / 4199

页数：13

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