Postfractionation for enhanced proteomic analyses: Routine electrophoretic methods increase the resolution of standard 2D-PAGE

被引：50

作者：

Butt, RH

Coorssen, JR

机构：

[1] Univ Calgary, Dept Physiol & Biophys, Calgary, AB, Canada

[2] Univ Calgary, Dept Biochem & Mol Biol, Hotchkiss Brain Inst, Fac Med, Calgary, AB, Canada

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 03期

关键词：

proteomics; membrane proteins; gradient gels; third separation; peptides;

D O I：

10.1021/pr050054d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we have addressed common issues of resolution in two-dimensional polyacrylamide gel electrophoresis (2DE) experiments including proteins 'stacked' at pH extremes, unresolved peptides migrating at the front of separation, and areas of the 2D gel obscured by high abundance proteins. Postfractionation, by selective application of well-established electrophoretic separations immediately following standard 2DE, yields markedly improved resolution in these traditional problem areas using no more specialized equipment or techniques than SDS-PAGE itself.

引用

页码：982 / 991

页数：10

共 37 条

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