Protective effect of melatonin against in vitro iron ions and 7 mT 50 Hz magnetic field-induced DNA damage in rat lymphocytes

被引：34

作者：

Jajte, J ^{[1
]}

Zmyslony, M ^{[1
]}

Palus, J ^{[1
]}

Dziubaltowska, E ^{[1
]}

Rajkowska, E ^{[1
]}

机构：

[1] Nofer Inst Occupat Med, PL-90950 Lodz, Poland

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2001年 / 483卷 / 1-2期

关键词：

50 Hz magnetic field; melatonin; DNA damage; comet assay; lymphocytes;

D O I：

10.1016/S0027-5107(01)00230-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have previously shown that simultaneous exposure of rat lymphocytes to iron ions and 50Hz magnetic field (NM) caused an increase in the number of cells with DNA strand breaks. Although the mechanism of MT-induced DNA damage is not known, we suppose that it involves free radicals. In the present study, to confirm our hypothesis, we have examined the effect of melatonin, an established free radicals scavenger, on DNA damage in rat peripheral blood lymphocytes exposed in vitro to iron ions and 50 Hz MY. The alkaline comet assay was chosen for the assessment of DNA damage. During pre-incubation, part of the cell samples were supplemented with melatonin (0.5 or 1.0 mM). The experiments were performed on the cell samples incubated for 3 h in Helmholtz coils at 7 mT 50 Hz NIF. During MF exposure, some samples were treated with ferrous chloride (FeCl2, 10 mug/ml), while the rest served as controls. A significant increase in the number of cells with DNA damage was found only after simultaneous exposure of lymphocytes to FeCl2 and 7 mT 50 Hz MF, compared to the control samples or those incubated with FeCl2 alone. However, when the cells were treated with melatonin and then exposed to iron ions and 50Hz MF, the number of damaged cells was significantly reduced, and the effect depended on the concentration of melatonin. The reduction reached about 50% at 0.5 mM and about 100% at 1.0 mM. Our results indicate that melatonin provides protection against DNA damage in rat lymphocytes exposed in vitro to iron ions and 50 Hz NM (7 mT). Therefore, it can be suggested that free radicals may be involved in 50 Hz magnetic field and iron ions-induced DNA damage in rat blood lymphocytes. The future experimental studies, in vitro and in vivo, should provide an answer to the question concerning the role of melatonin in the free radical processes in the power frequency magnetic field. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：57 / 64

页数：8

共 39 条

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