Analysis of saikosaponins in Bupleuri Radix and Caihu-shugan-san using reversed-phase HPLC with pulsed amperometric detection

被引：12

作者：

Kwon, Ha-Jeong ^{[2
]}

Sim, Hee-Jung ^{[1
]}

Lee, Sa-Im ^{[1
]}

Lee, Yong-Moon ^{[3
]}

Lee, Je-Hyun ^{[4
]}

Park, Yong-Duk ^{[2
]}

Hong, Seon-Pyo ^{[1
]}

机构：

[1] Kyung Hee Univ, Dept Oriental Pharmaceut Sci, Kyung Hee EW Pharmaceut Res Inst, Coll Pharm, Seoul 130701, South Korea

[2] Kyung Hee Univ, Dept Prevent & Social Dent, Grad Sch, Seoul 130701, South Korea

[3] Chungbuk Natl Univ, Coll Pharm, CBITRC, Chonju, South Korea

[4] Dongguk Univ, Dept Korean Med, Geongju, South Korea

来源：

JOURNAL OF SEPARATION SCIENCE | 2011年 / 34卷 / 06期

关键词：

Pulsed amperometric detection; Reversed-phase HPLC; Saikosaponins; Stability; ANION-EXCHANGE CHROMATOGRAPHY; LIQUID-CHROMATOGRAPHY; APOPTOTIC MECHANISM; CELLS; INHIBITION; QUANTIFICATION; SUPPRESSION; ACTIVATION; GROWTH; INJURY;

D O I：

10.1002/jssc.201000784

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A simple and sensitive reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD) method was developed to determine the saikosaponin content in Bupleuri Radix or Caihu-shugan-san. Four saikosaponins in Bupleuri Radix and Caihu-shugan-san were extracted with a 6:4 solution of 10 mM sodium phosphate buffer (pH 8)/100% ethanol. Pulsed amperometric detection of carbohydrates in four major saikosaponins was highly sensitive when used with a water-acetonitrile gradient on an alkaline RP column with a post-column delivery system. The limits of detection (S/N = 3) and of quantification (S/N = 10) of saikosaponins were 0.01-0.02 and 0.03-0.05 mu g/mL, respectively. The intra- and inter-day precision (RSDs) were each < 9.7% and the average recoveries were 95.0-97.6% in Bupleuri Radix. This method can be used to analyze saikosaponins in Bupleuri Radix and Caihu-shugan-san.

引用

页码：651 / 658

页数：8

共 34 条

[1]

Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the Chinese multiherb remedy Xiaochaihu-tang using liquid chromatographic tandem mass spectrometry [J].