Pulsatilla saponin A induces differentiation in acute myeloid leukemia in vitro

被引：20

作者：

Wang, Tong ^{[1
,2
]}

Gong, Fang ^{[1
,2
]}

Zhang, Ri ^{[2
]}

Cen, Jiannong ^{[2
]}

Dai, Lan ^{[2
]}

Zhu, Mingqing ^{[2
]}

Qi, Xiaofei ^{[1
,2
,3
]}

机构：

[1] Jiangsu Inst Hematol, Minist Hlth, Key Lab Thrombosis & Hemostasis, Cyrus Tang Hematol Ctr,Collaborat Innovat Ctr Hem, Suzhou 215006, Peoples R China

[2] Soochow Univ, Affiliated Hosp 1, Dept Hematol, Suzhou 215006, Peoples R China

[3] Soochow Univ, Affiliated Hosp 1, Dept Urol, Suzhou 215006, Peoples R China

来源：

HEMATOLOGY | 2016年 / 21卷 / 03期

基金：

美国国家科学基金会;

关键词：

Pulsatilla saponin A; Acute myeloid leukemia; Differentiation; CHINENSIS BUNGE REGEL; COLON-CANCER CELLS; MEGAKARYOCYTIC DIFFERENTIATION; K562; CELLS; PATHWAY; PROLIFERATION; APOPTOSIS; XENOGRAFT; GROWTH;

D O I：

10.1080/10245332.2015.1101967

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Objectives: To identify whether Pulsatilla saponin A (PsA), an active molecule extracted from Pulsatilla chinensis regel, can induce acute myeloid leukemia (AML) cells differentiate. Methods: PsA was isolated from P. chinensis, and its effects of differentiation induction on both AML cell lines and the primary leukemia cells were investigated. Results: Compared with the untreated control, PsA induced the differentiation of U937 cells, K562 cells and HL-60 cells, represented as the increased CD15+ cells in a dose-and time-dependent manner in all the three AML cell lines, after PsA treatment. As the same time, the cell morphology of these AML cells was changed correspondingly; the cytoplasm/nuclei ratio was increased, basophilic cytoplasm was decreased, and eccentric nucleus and granules were also observed. Also, the same effects of differentiation induction by PSA were confirmed in the primary leukemia cells. However, the specific MEK/ERK inhibitor U0126 effectively abrogated the differentiation induced by PsA in vitro. Conclusions: PsA can modify the differentiation activity of AML cells, probably though the MEK/ERK signaling pathway.

引用

页码：182 / 186

页数：5

共 13 条

[1]

Silver complexation and tandem mass spectrometry for differentiation of triterpenoid saponins from the roots of Pulsatilla chinensis (Bunge) Regel [J].