The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus

被引:107
作者
Draviam, VM
Orrechia, S
Lowe, M
Pardi, R
Pines, J
机构
[1] Univ Cambridge, Dept Zool, Wellcome CRC Inst, Cambridge CB2 1QR, England
[2] Vita Salute Univ, Sch Med, San Raffaele Sci Inst, I-20132 Milan, Italy
[3] Univ Manchester, Sch Biol Sci, Div Biochem, Manchester M13 9PT, Lancs, England
关键词
cyclin; CDK; mitosis; protein kinase; Golgi apparatus;
D O I
10.1083/jcb.152.5.945
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In this paper. we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization of the microtubules, and disassembly of the nuclear lamina and of the Golgi apparatus, while it restricts cyclin B2-CDK1 to disassembly of the Golgi apparatus. We identify the region of cyclin B2 responsible for its localization and show that this will direct cyclin B1 to the Golgi apparatus and confer upon it the more limited properties of cyclin B2. Equally, directing cyclin B2 to the cytoplasm with the NH2 terminus of cyclin B1 confers the broader properties of cyclin B1. Furthermore. we show that the disassembly of the Golgi apparatus initiated by either mitotic cyclin-CDK complex does not require mitogen-activated protein kinase kinase (MEK) activity.
引用
收藏
页码:945 / 958
页数:14
相关论文
共 44 条
  • [31] STRUCTURE AND GROWTH-DEPENDENT REGULATION OF THE HUMAN CYCLIN B1 PROMOTER
    PIAGGIO, G
    FARINA, A
    PERROTTI, D
    MANNI, I
    FUSCHI, P
    SACCHI, A
    GAETANO, C
    [J]. EXPERIMENTAL CELL RESEARCH, 1995, 216 (02) : 396 - 402
  • [32] CYCLINS AND CYCLIN-DEPENDENT KINASES - THEME AND VARIATIONS
    PINES, J
    [J]. ADVANCES IN CANCER RESEARCH, VOL 66, 1995, 66 : 181 - 212
  • [33] HUMAN CYCLIN-A AND CYCLIN-B1 ARE DIFFERENTIALLY LOCATED IN THE CELL AND UNDERGO CELL-CYCLE DEPENDENT NUCLEAR TRANSPORT
    PINES, J
    HUNTER, T
    [J]. JOURNAL OF CELL BIOLOGY, 1991, 115 (01) : 1 - 17
  • [34] Four-dimensional control of the cell cycle
    Pines, J
    [J]. NATURE CELL BIOLOGY, 1999, 1 (03) : E73 - E79
  • [35] Evolving ideas about cyclins
    Roberts, JM
    [J]. CELL, 1999, 98 (02) : 129 - 132
  • [36] Crystal structure of the p27(Kip1) cyclin-dependent-kinase inhibitor bound to the cyclin A Cdk2 complex
    Russo, AA
    Jeffrey, PD
    Patten, AK
    Massague, J
    Pavletich, NP
    [J]. NATURE, 1996, 382 (6589) : 325 - 331
  • [37] p21(CIP1) and Cdc25A: Competition between an inhibitor and an activator of cyclin-dependent kinases
    Saha, P
    Eichbaum, Q
    Silberman, ED
    Mayer, BJ
    Dutta, A
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (08) : 4338 - 4345
  • [38] Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A
    Schulman, BA
    Lindstrom, DL
    Harlow, E
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) : 10453 - 10458
  • [39] Partitioning of the Golgi apparatus during mitosis in living HeLa cells
    Shima, DT
    Haldar, K
    Pepperkok, R
    Watson, R
    Warren, G
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 137 (06) : 1211 - 1228
  • [40] Stern B, 1996, TRENDS GENET, V12, P345