The mouse CCR2 gene is regulated by two promoters that are responsive to plasma cholesterol and peroxisome proliferator-activated receptor γ ligands

被引：29

作者：

Chen, YM ^{[1
]}

Green, SR ^{[1
]}

Ho, J ^{[1
]}

Li, A ^{[1
]}

Almazan, F ^{[1
]}

Quehenberger, O ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 332卷 / 01期

关键词：

CCR2; chemokines; promoter; gene expression; cholesterol; PPAR gamma; atherosclerosis;

D O I：

10.1016/j.bbrc.2005.04.110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously shown that the expression of monocyte CCR2, the receptor for monocyte chemoattractant protein-1, is induced by plasma cholesterol. The present study examines the mechanisms that regulate monocyte CCR2 expression in hypercholesterolemia using a mouse model. Our data demonstrate that in the Mouse, CCR2 expression in circulating monocytes is controlled by two promoters P1 and P2. The two distinct transcripts, which encode the same protein, are produced by alternative splicing in the 5'-untranslated region. Both promoters are constitutively active, but only P2 is stimulated by cholesterol. However, both promoters are repressed by peroxisome proliferator-activated receptor gamma. (c) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：188 / 193

页数：6

相关论文

共 24 条

[11]

Han KH, 1999, J LIPID RES, V40, P1053

[12] Chemokine receptor CCR2 expression and monocyte chemoattractant protein-1-mediated chemotaxis in human monocytes - A regulatory role for plasma LDL [J].

Han, KH ;

Tangirala, RK ;

Green, SR ;

Quehenberger, O .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1998, 18 (12) :1983-1991

[13] Ligands for peroxisome proliferator-activated receptor inhibit monocyte CCR2 expression stimulated by plasma lipoproteins [J].

Han, KH ;

Quehenberger, O .

TRENDS IN CARDIOVASCULAR MEDICINE, 2000, 10 (05) :209-216

[14] Oxidized LDL reduces monocyte CCR2 expression through pathways involving peroxisome proliferator-activated receptor γ [J].

Han, KH ;

Chang, MK ;

Boullier, A ;

Green, SR ;

Li, A ;

Glass, CK ;

Quehenberger, O .

JOURNAL OF CLINICAL INVESTIGATION, 2000, 106 (06) :793-802

[15] Expression and function of PPARγ in rat and human vascular smooth muscle cells [J].

Law, RE ;

Goetze, S ;

Xi, XP ;

Jackson, S ;

Kawano, Y ;

Demer, L ;

Fishbein, MC ;

Meehan, WP ;

Hsueh, WA .

CIRCULATION, 2000, 101 (11) :1311-1318

[16] Peroxisome proliferator-activate inhibit development of atherosclerosis in LDL receptor-deficient mice [J].

Li, AC ;

Brown, KK ;

Silvestre, MJ ;

Willson, TM ;

Palinski, W ;

Glass, CK .

JOURNAL OF CLINICAL INVESTIGATION, 2000, 106 (04) :523-531

[17] Potent inhibitory effect of troglitazone on carotid arterial wall thickness in type 2 diabetes [J].

Minamikawa, J ;

Tanaka, S ;

Yamauchi, M ;

Inoue, D ;

Koshiyama, H .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1998, 83 (05) :1818-1820

[18] The human CC chemokine receptor 5 (CCR5) gene -: Multiple transcripts with 5′-end heterogeneity, dual promoter usage, and evidence for polymorphisms within the regulatory regions and noncoding exons [J].

Mummidi, S ;

Ahuja, SS ;

McDaniel, BL ;

Ahuja, SK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (49) :30662-30671

[19] Expression of the peroxisome proliferator-activated receptor γ (PPARγ) in human atherosclerosis and regulation in macrophages by colony stimulating factors and oxidized low density lipoprotein [J].

Ricote, M ;

Huang, J ;

Fajas, L ;

Li, A ;

Welch, J ;

Najib, J ;

Witztum, JL ;

Auwerx, J ;

Palinski, W ;

Glass, CK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (13) :7614-7619

[20] FATTY STREAK EXPANSION AND MATURATION IN WATANABE HERITABLE HYPERLIPEMIC AND COMPARABLY HYPERCHOLESTEROLEMIC FAT-FED RABBITS [J].

ROSENFELD, ME ;

TSUKADA, T ;

CHAIT, A ;

BIERMAN, EL ;

GOWN, AM ;

ROSS, R .

ARTERIOSCLEROSIS, 1987, 7 (01) :24-34