Proanthocyanidin from grape seeds inactivates the PI3-kinase/PKB pathway and induces apoptosis in a colon cancer cell line

被引：131

作者：

Engelbrecht, A. -M. ^{[1
]}

Mattheyse, M. ^{[1
]}

Ellis, B. ^{[1
]}

Loos, B. ^{[1
]}

Thomas, M. ^{[1
]}

Smith, R. ^{[1
]}

Peters, S. ^{[1
]}

Smith, C. ^{[1
]}

Myburgh, K. ^{[1
]}

机构：

[1] Univ Stellenbosch, Dept Physiol Sci, ZA-7600 Stellenbosch, South Africa

来源：

CANCER LETTERS | 2007年 / 258卷 / 01期

关键词：

PI3-k; PKB/Akt; apoptosis; colon cancer;

D O I：

10.1016/j.canlet.2007.08.020

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of this investigation was to evaluate the chemopreventative/antiproliferative potential of a grape seed proanthocyanidin extract (GSPE) against colon cancer cells (CaCo2 cells) and to investigate its mechanism of action. GSPE (10-100 mu g/ml) significantly inhibited cell viability and increased apoptosis in CaCo2 cells, but did not alter viability in the normal colon cell line (NCM460). The increased apoptosis observed in GSPE-treated CaCo2 cells correlated with an attenuation of PI3-kinase (p 110 and p85 subunits) and decreased PKB Ser 473 phosphorylation. GSPE might thus exert its beneficial effects by means of increased apoptosis and suppression of the important PI3-kinase survival-related pathway. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：144 / 153

页数：10

共 42 条

[11] Dihydroxyphenylethanol induces apoptosis by activating serine/threonine protein phosphatase PP2A and promotes the endoplasmic reticulum stress response in human colon carcinoma cells [J].

Guichard, Cecile ;

Pedruzzi, Eric ;

Fay, Michele ;

Marie, Jean-Claude ;

Braut-Boucher, Francoise ;

Daniel, Fanny ;

Grodet, Alain ;

Gougerot-Pocidalo, Marie-Anne ;

Chastre, Eric ;

Kotelevets, Larissa ;

Lizard, Gerard ;

Vandewalle, Alain ;

Driss, Fathi ;

Ogier-Denis, Eric .

CARCINOGENESIS, 2006, 27 (09) :1812-1827

[12] Colorectal cancer prevention and treatment by inhibition of cyclooxygenase-2 [J].

Gupta, RA ;

DuBois, RN .

NATURE REVIEWS CANCER, 2001, 1 (01) :11-21

[13] The hallmarks of cancer [J].

Hanahan, D ;

Weinberg, RA .

CELL, 2000, 100 (01) :57-70

[14] Lysophosphatidylcholine induces apoptotic and non-apoptotic death in vascular smooth muscle cells: in comparison with oxidized LDL [J].

Hsieh, CC ;

Yen, MH ;

Liu, HW ;

Lau, YT .

ATHEROSCLEROSIS, 2000, 151 (02) :481-491

[15] TSC2 mediates cellular energy response to control cell growth and survival [J].

Inoki, K ;

Zhu, TQ ;

Guan, KL .

CELL, 2003, 115 (05) :577-590

[16] Specific binding of the Akt-1 protein kinase to phosphatidylinositol 3,4,5-trisphosphate without subsequent activation [J].

James, SR ;

Downes, CP ;

Gigg, R ;

Grove, SJA ;

Holmes, AB ;

Alessi, DR .

BIOCHEMICAL JOURNAL, 1996, 315 :709-713

[17] Cancer statistics, 2005 [J].

Jemal, A ;

Murray, T ;

Ward, E ;

Samuels, A ;

Tiwari, RC ;

Ghafoor, A ;

Feuer, EJ ;

Thun, MJ .

CA-A CANCER JOURNAL FOR CLINICIANS, 2005, 55 (01) :10-30

[18]

Joshi S. S., 2001, CURR PHARM BIOTECHNO, V2, P2187

[19] The PI 3-kinase/Akt signaling pathway delivers an anti-apoptotic signal [J].

Kennedy, SG ;

Wagner, AJ ;

Conzen, SD ;

Jordan, J ;

Bellacosa, A ;

Tsichlis, PN ;

Hay, N .

GENES & DEVELOPMENT, 1997, 11 (06) :701-713

[20] Lessons from hereditary colorectal cancer [J].

Kinzler, KW ;

Vogelstein, B .

CELL, 1996, 87 (02) :159-170

← 1 2 3 4 5 →