Ultrafast Surface Enhanced Resonance Raman Scattering Detection in Droplet-Based Microfluidic Systems

被引:94
作者
Cecchini, Michael P. [2 ]
Hong, Jongin [3 ]
Lim, Chaesung [4 ]
Choo, Jaebum [4 ]
Albrecht, Tim [1 ]
deMello, Andrew J. [1 ]
Edel, Joshua B. [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Mat, London SW7 2AZ, England
[3] Chung Ang Univ, Dept Chem, Seoul 156756, South Korea
[4] Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea
基金
新加坡国家研究基金会; 英国工程与自然科学研究理事会;
关键词
ELECTROCHEMICAL DETECTION; LATEST DEVELOPMENTS; MICRODROPLETS; KINETICS; MICRO;
D O I
10.1021/ac103329b
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The development of ultrafast Raman-based detection is one of the most interesting challenges underpinning the application of droplet based microfluidics. Herein, we describe the use of surface-enhanced resonance Raman spectroscopy (SERRS) with submillisecond time resolution as a powerful detection tool in microdroplet reactors. Individual droplets containing silver nanoparticle aggregates functionalized with Raman reporters are interrogated and characterized by full spectra acquisitions with high spatial resolution in real time. Whereas previous works coupling SERRS with droplet-based microfluidics acquire a single spectrum over single or multiple droplets, we build upon these results by increasing our temporal resolution by 2 orders of magnitude. This allows us to interrogate multiple points within one individual droplet. The SERRS signals emitted from the aggregates are utilized to access the influence of flow rate on droplet size and throughput. Accordingly, our approach allows for high-throughput analysis that facilitates the study of other biological assays or molecular interactions.
引用
收藏
页码:3076 / 3081
页数:6
相关论文
共 48 条
[1]   Quantitative online detection of low-concentrated drugs via a SERS microfluidic system [J].
Ackermann, Katrin R. ;
Henkel, Thomas ;
Popp, Juergen .
CHEMPHYSCHEM, 2007, 8 (18) :2665-2670
[2]   Ultrahigh-throughput screening in drop-based microfluidics for directed evolution [J].
Agresti, Jeremy J. ;
Antipov, Eugene ;
Abate, Adam R. ;
Ahn, Keunho ;
Rowat, Amy C. ;
Baret, Jean-Christophe ;
Marquez, Manuel ;
Klibanov, Alexander M. ;
Griffiths, Andrew D. ;
Weitz, David A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (09) :4004-4009
[3]   Latest Developments in Micro Total Analysis Systems [J].
Arora, Arun ;
Simone, Giuseppina ;
Salieb-Beugelaar, Georgette B. ;
Kim, Jung Tae ;
Manz, Andreas .
ANALYTICAL CHEMISTRY, 2010, 82 (12) :4830-4847
[4]   A double droplet trap system for studying mass transport across a droplet-droplet interface [J].
Bai, Yunpeng ;
He, Ximin ;
Liu, Dingsheng ;
Patil, Santoshkumar N. ;
Bratton, Dan ;
Huebner, Ansgar ;
Hollfelder, Florian ;
Abell, Chris ;
Huck, Wilhelm T. S. .
LAB ON A CHIP, 2010, 10 (10) :1281-1285
[5]   Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity [J].
Baret, Jean-Christophe ;
Miller, Oliver J. ;
Taly, Valerie ;
Ryckelynck, Michael ;
El-Harrak, Abdeslam ;
Frenz, Lucas ;
Rick, Christian ;
Samuels, Michael L. ;
Hutchison, J. Brian ;
Agresti, Jeremy J. ;
Link, Darren R. ;
Weitz, David A. ;
Griffiths, Andrew D. .
LAB ON A CHIP, 2009, 9 (13) :1850-1858
[6]   High-temperature microfluidic synthesis of CdSe nanocrystals in nanoliter droplets [J].
Chan, EM ;
Alivisatos, AP ;
Mathies, RA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (40) :13854-13861
[7]   Chemistry and Biology in Femtoliter and Picoliter Volume Droplets [J].
Chiu, Daniel T. ;
Lorenz, Robert M. .
ACCOUNTS OF CHEMICAL RESEARCH, 2009, 42 (05) :649-658
[8]   Control and detection of chemical reactions in microfluidic systems [J].
deMello, Andrew J. .
NATURE, 2006, 442 (7101) :394-402
[9]   Drug screening - Beyond the bottleneck [J].
Dove, A .
NATURE BIOTECHNOLOGY, 1999, 17 (09) :859-863
[10]   Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online [J].
Fidalgo, Luis M. ;
Whyte, Graeme ;
Ruotolo, Brandon T. ;
Benesch, Justin L. P. ;
Stengel, Florian ;
Abell, Chris ;
Robinson, Carol V. ;
Huck, Wilhelm T. S. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (20) :3665-3668