Detergent-resistant microdomains offer no refuge for proteins phosphorylated by the IgE receptor

被引:27
作者
Peirce, M [1 ]
Metzger, H [1 ]
机构
[1] NIAMS, Arthritis & Rheumatism Branch, Sect Chem Immunol, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1074/jbc.M005819200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When the high affinity receptor for IgE and related receptors become aggregated, they emigrate to specialized microdomains of the plasma membrane that are enriched in certain lipids and lipid-anchored proteins. Among the latter are the kinases that initiate signaling cascade(s) by phosphorylating the receptors. In studying the IgE receptor, we explored whether, in addition to their potential role in enhancing the initiation of signaling by the kinase(s), the microdomains might augment the stimulation by excluding phosphatases. In vitro assessment of phosphatase activity, using either a relevant or irrelevant substrate, suggested that the microdomains were deficient in phosphatase activity, but, in vivo, proteins confined to the microdomains were found to be no less vulnerable to dephosphorylation than those outside such domains. In the course of our experiments, we observed that the procedures routinely used to isolate the detergent-resistant domains dissociated the receptor for IgE, thereby artificially accentuating the observed preferential distribution of phosphorylated subunits in the microdomains.
引用
收藏
页码:34976 / 34982
页数:7
相关论文
共 43 条
[1]  
ADAMCZEWSKI M, 1992, J BIOL CHEM, V267, P18126
[2]  
ALBER G, 1992, J IMMUNOL, V149, P2428
[3]  
ALCARAZ G, 1984, J BIOL CHEM, V259, P4922
[4]   Src family-selective tyrosine kinase inhibitor, PP1, inhibits both Fc epsilon RI- and Thy-1-mediated activation of rat basophilic leukemia cells [J].
Amoui, M ;
Draber, P ;
Draberova, L .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (08) :1881-1886
[5]   How does the plasma membrane participate in cellular signaling by receptors for immunoglobulin E? [J].
Baird, B ;
Sheets, ED ;
Holowka, D .
BIOPHYSICAL CHEMISTRY, 1999, 82 (2-3) :109-119
[6]   IGE-INDUCED HISTAMINE-RELEASE FROM RAT BASOPHILIC LEUKEMIA-CELL LINES - ISOLATION OF RELEASING AND NON-RELEASING CLONES [J].
BARSUMIAN, EL ;
ISERSKY, C ;
PETRINO, MG ;
SIRAGANIAN, RP .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1981, 11 (04) :317-323
[7]   COMPLETE STRUCTURE AND EXPRESSION IN TRANSFECTED CELLS OF HIGH-AFFINITY IGE RECEPTOR [J].
BLANK, U ;
RA, C ;
MILLER, L ;
WHITE, K ;
METZGER, H ;
KINET, JP .
NATURE, 1989, 337 (6203) :187-189
[8]   Interaction of phosphorylated Fc epsilon RI gamma immunoglobulin receptor tyrosine activation motif-based peptides with dual and single SH2 domains of p72(syk) - Assessment of binding parameters and real, time binding kinetics [J].
Chen, T ;
Repetto, B ;
Chizzonite, R ;
Pullar, C ;
Burghardt, C ;
Dharm, E ;
Zhao, AC ;
Carroll, R ;
Nunes, P ;
Basu, M ;
Danho, W ;
Visnick, M ;
Kochan, J ;
Waugh, D ;
Gilfillan, AM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (41) :25308-25315
[9]  
EISEMAN E, 1992, NATURE, V355, P78
[10]  
FEWTRELL C, 1980, J IMMUNOL, V125, P701