Chemical composition and anti-Acanthamoeba activity of Melaleuca styphelioides essential oil

被引：12

作者：

Albouchi, Ferdaous ^{[1
,2
]}

Sifaoui, Ines ^{[1
,3
]}

Reyes-Batlle, Maria ^{[3
]}

Lopez-Arencibia, Atteneri ^{[3
]}

Pinero, Jose E. ^{[3
]}

Lorenzo-Morales, Jacob ^{[3
]}

Abderrabba, Manef ^{[1
]}

机构：

[1] Univ Carthage, Lab Mat Mol & Applicat, IPEST, BP 51, La Marsa 2070, Tunisia

[2] Fac Sci Bizerte, Jarzouna 7021, Bizerte, Tunisia

[3] Univ La Laguna, Univ Inst Trop Dis & Publ Hlth, Avda Francisco Sanchez S-N,Campus Anchieta, Tenerife 38271, Canary Islands, Spain

来源：

EXPERIMENTAL PARASITOLOGY | 2017年 / 183卷

关键词：

Melaleuca styphelioides; Essential oil; Anti-Acanthameoba activity; ENTAMOEBA-HISTOLYTICA; TROPHOZOITE STAGE; SESQUITERPENE; ANTIOXIDANT; NEFF;

D O I：

10.1016/j.exppara.2017.10.014

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Acanthamoeba infections cause serious humans diseases, such as amoebic keratitis and granulomatous amoebic encephalitis. Melaleuca essential oil has been reported to be effective in treating bacterial and fungal infections. However, the anti-parasitic effects of this essential oil are not well studied. In the present study, we first characterized the composition of the essential oil, extracted from the Tunisian Melaleuca styphelioides leaves, and then tested its effect on the Acanthamoeba castellanii Neff. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the major common compounds were Caryophyllene oxide (23.42%), Spathulenol (20.5%), Isoaromadendrene epoxide (7.45%), Ledol (5.98%), alpha-Pinene (3.82%), Isopinocarveol (2.18%). Our data also showed that M. styphelioides essential oil inhibited the growth of Acanthamoeba with an IC50 value of 69.03 +/- 9.17 mu g/ml. This work suggests M. styphelioides essential oil as a potential anti amoeba drug. Nevertheless, further studies are still needed to further verify the cytotoxicity of the studied oil on human macrophages and check its applicability to treat Acanthamoeba infections in vivo. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：104 / 108

页数：5

共 24 条

[1]

Astani A, 2011, J EVID BASED COMPLEM, V44, P8

[2]

Attman PM, 1988, AJP, V69, P276

[3] Inhibitory effects of Iranian Thymus vulgaris extracts on in vitro growth of Entamoeba histolytica [J].