Generation of CD1 tetramers as a tool to monitor glycolipid-specific T cells

被引:12
作者
Gadola, SD
Karadimitris, A
Zaccai, NR
Salio, M
Dulphy, N
Shepherd, D
Jones, EY
Cerundolo, V
机构
[1] Univ Oxford, Weatherall Inst Mol Med, Tumor Immunol Unit, Oxford OX3 9DS, England
[2] Univ Oxford, Receptor Struct Res Grp, Oxford OX3 9DS, England
关键词
CDI; glycolipids; antigen presentation; T lymphocytes; alpha-galactosylceramide;
D O I
10.1098/rstb.2003.1267
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
CD I molecules are beta(2)m-associated HLA class-I-like glycoproteins which have the unique ability to present glycolipid and phospholipid antigens to specific T lymphocytes. To study the biology of CD1 and its role in human disease we developed novel techniques for generation of recombinant CD1/lipid complexes by in vitro refolding. Fluorescent tetrameric complexes made from soluble recombinant CD1d/alpha-galactosylceramide complexes allowed highly sensitive and specific ex vivo and in vitro detection and functional characterization of novel human T-lymphocyte populations. Furthermore, protein crystals were obtained from soluble recombinant CD1b/beta(2)m-proteins loaded either with phosphatidylinositol or ganglioside GM2, which led to the first atomic structure determination of a CD1/lipid complex. The analysis of these crystal structures clarified how CD1b molecules can bind lipid ligands of different size, and revealed a broader spectrum of potential CD1b ligands than previously predicted.
引用
收藏
页码:875 / 877
页数:3
相关论文
共 25 条
  • [1] Murine natural killer cells contribute to the granulomatous reaction caused by mycobacterial cell walls
    Apostolou, I
    Takahama, Y
    Belmant, C
    Kawano, T
    Huerre, M
    Marchal, G
    Cui, J
    Taniguchi, M
    Nakauchi, H
    Fournié, JJ
    Kourilsky, P
    Gachelin, G
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (09) : 5141 - 5146
  • [2] RECOGNITION OF A LIPID ANTIGEN BY CD1-RESTRICTED ALPHA-BETA(+) T-CELLS
    BEEKMAN, EM
    PORCELLI, SA
    MORITA, CT
    BEHAR, SM
    FURLONG, ST
    BRENNER, MB
    [J]. NATURE, 1994, 372 (6507) : 691 - 694
  • [3] In vivo identification of glycolipid antigen-specific T cells using fluorescent CD1d tetramers
    Benlagha, K
    Weiss, A
    Beavis, A
    Teyton, L
    Bendelac, A
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (11) : 1895 - 1903
  • [4] A thymic precursor to the NK T cell lineage
    Benlagha, K
    Kyin, T
    Beavis, A
    Teyton, L
    Bendelac, A
    [J]. SCIENCE, 2002, 296 (5567) : 553 - 555
  • [5] Davodeau F, 1997, J IMMUNOL, V158, P5603
  • [6] Vα24-JαQ-independent, CD1d-restricted recognition of α-galactosylceramide by human CD4+ and CD8αβ+ T lymphocytes
    Gadola, SD
    Dulphy, N
    Salio, M
    Cerundolo, V
    [J]. JOURNAL OF IMMUNOLOGY, 2002, 168 (11) : 5514 - 5520
  • [7] Structure of human CD1b with bound ligands at 2.3 Å, a maze for alkyl chains
    Gadola, SD
    Zaccai, NR
    Harlos, K
    Shepherd, D
    Castro-Palomino, JC
    Ritter, G
    Schmidt, RR
    Jones, EY
    Cerundolo, V
    [J]. NATURE IMMUNOLOGY, 2002, 3 (08) : 721 - 726
  • [8] Murine CD1d-restricted T cell recognition of cellular lipids
    Gumperz, JE
    Roy, C
    Makowska, A
    Lum, D
    Sugita, M
    Podrebarac, T
    Koezuka, Y
    Porcelli, SA
    Cardell, S
    Brenner, MB
    Behar, SM
    [J]. IMMUNITY, 2000, 12 (02) : 211 - 221
  • [9] Human CD1d-glycolipid tetramers generated by in vitro oxidative refolding chromatography
    Karadimitris, A
    Gadola, S
    Altamirano, M
    Brown, D
    Woolfson, A
    Klenerman, P
    Chen, JL
    Koezuka, Y
    Roberts, IAG
    Price, DA
    Dusheiko, G
    Milstein, C
    Fersht, A
    Luzzatto, L
    Cerundolo, V
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) : 3294 - 3298
  • [10] Tracking T cells with tetramers: New tales from new tools
    Klenerman, P
    Cerundolo, V
    Dunbar, RR
    [J]. NATURE REVIEWS IMMUNOLOGY, 2002, 2 (04) : 263 - 272