Induction of cell cycle arrest and apoptosis by the ethyl acetate fraction of Kalopanax pictus leaves in human colon cancer cells

被引：49

作者：

Hu, Weicheng ^{[1
]}

Lee, Seok Ki ^{[2
]}

Jung, Mee Jung ^{[1
]}

Heo, Seong-Il ^{[1
]}

Hur, Jang Hyun ^{[3
]}

Wang, Myeong-Hyeon ^{[1
]}

机构：

[1] Kangwon Natl Univ, Dept Med Biotechnol, Coll Biomed Sci, Chunchon 200701, Kangwon Do, South Korea

[2] Kangwon Natl Univ, Dept Otolaryngol, Coll Med, Chunchon 200701, Kangwon Do, South Korea

[3] Kangwon Natl Univ, Div Environm Biol, Chunchon 200701, Kangwon Do, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 23期

关键词：

Apoptosis; Bcl-2; Cell cycle; Kalopanax pictus leaf; Reactive oxygen species; G2/M PHASE; LINES; MITOCHONDRIA; CASPASE-3; PATHWAYS; EXTRACT; GROWTH; DEATH; BCL-2; MCF-7;

D O I：

10.1016/j.biortech.2010.06.091

中图分类号：

S2 [农业工程];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Kalopanax pictus is a deciduous tree used in traditional medicine; its leaves are also consumed as a vegetable. In this study, the ethyl acetate fraction of K. pictus leaves (EFK) was tested in vitro for anticancer activity against four cell lines: human colon cancer (HT-29) cells, human stomach cancer (NCI-N87) cells, human breast cancer (MDA-MB231) cells, and mouse melanoma (B16F1) cells. Results indicated that EFK showed the most potent tumor selective growth inhibitory activity against HT-29 cells with less cytotoxic effect on normal cell lines. Cytotoxicity of EFK on HT-29 cells was associated mainly with cell chromatin condensation, DNA fragmentation, and loss of membrane phospholipid asymmetry with appearance of G2/M phase arrest. Cell death induced by EFK displayed features characteristic of apoptosis, and was associated with generation of reactive oxygen species (ROS) and increase of Bax/Bcl-2 ratio. These findings suggest that K. pictus leaves have anticancer properties and may be valuable for application in pharmaceutical industry. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：9366 / 9372

页数：7

共 35 条

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Mitochondrial membrane permeabilization: the sine qua non for cell death [J].