A validated 1H NMR method for the determination of the degree of deacetylation of chitosan

被引：556

作者：

Lavertu, M

Xia, Z

Serreqi, AN

Berrada, M

Rodrigues, A

Wang, D

Buschmann, MD

Gupta, A

机构：

[1] Ecole Polytech, Inst Biomed & Chem Engn, Dept Chem Engn, Montreal, PQ H3C 3A7, Canada

[2] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

[3] Bio Syntech Canada Inc, Montreal, PQ H7V 4B3, Canada

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2003年 / 32卷 / 06期

关键词：

chitosan; chitin; pH-sensitive; IR; H-1; NMR; degree of deacetylation;

D O I：

10.1016/S0731-7085(03)00155-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of the degree of deacetylation (DDA) of chitosan by H-1 NMR spectroscopy has been formally validated. Chitosans with DDA ranging from 48 to 100% have been used for the validation. The method is found to be simple, rapid and more precise than other known techniques like IR or titration for %DDA measurements. The precision, ruggedness, robustness, specificity, stability and accuracy of the technique are discussed in this paper. (C) 2003 Published by Elsevier B.V.

引用

页码：1149 / 1158

页数：10

共 19 条

[1] STUDIES ON CHITOSAN .1. DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITOSAN BY ULTRAVIOLET SPECTROPHOTOMETRY AND GEL-PERMEATION CHROMATOGRAPHY [J].