Hyperthermia-induced apoptosis and the inhibition of DNA laddering by zinc supplementation and withdrawal of calcium and magnesium in suspension culture of tobacco cells

被引：23

作者：

Chen, H ^{[1
]}

Yan, C ^{[1
]}

Jiang, X ^{[1
]}

Dai, YR ^{[1
]}

机构：

[1] Tsing Hua Univ, Dept Biol, Beijing 100084, Peoples R China

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 1999年 / 55卷 / 02期

关键词：

tobacco cell; heat shock; apoptosis; DNA laddering; TUNEL; zinc;

D O I：

10.1007/s000180050292

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present gaper we report examination of stereotypic hallmarks of apoptosis in heat-treated tobacco cells. Hyperthermia (44 degrees C, 4 h) caused apoptosis in 53.6% of cells when assayed 24 h after heat treatment. The induction of apoptosis by heat treatment was confirmed by flow cytometric assay. Cytological observations revealed condensation of the cytoplasm and nucleus, as well as nuclear collapse. DNA ladders were observed in DNA extracted from heat-treated cells, whereas DNA from control cells remained undegraded. The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay revealed that 51.8% of the heat-treated cells (44 degrees C, 4 h) show positive reaction after a 24-h recovery. When cells were cultured in a medium supplemented with 0.4-5.0 mM ZnSO4, internucleosomal DNA fragmentation induced by heat shock was completely negated. Strikingly, when cells were cultured in Ca2+ and/or Mg2+ free medium for 44 h followed by heat treatment, DNA laddering was not observed. The results suggest hyperthermia-induced apoptosis and a correlation between the regulation of endonucleases and heat shock signal in apoptotic tobacco cells.

引用

页码：303 / 309

页数：7

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