The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase

被引：25

作者：

Cantoni, R

Branzoni, M

Labò, M

Rizzi, M

Riccardi, G

机构：

[1] Univ Pavia, Dipartimento Genet & Microbiol, I-27100 Pavia, Italy

[2] Univ Turin, Dipartimento Sci & Tecnol Farmaco, I-10125 Turin, Italy

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 12期

关键词：

D O I：

10.1128/JB.180.12.3218-3221.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD(+) synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His(6)-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCP428.08 gene of M. tuberculosis is the structural gene for NAD(+) synthetase.

引用

页码：3218 / 3221

页数：4

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