Growth factors regulation of rabbit sodium-dependent neutral amino acid transporter ATB0 and oligopeptide transporter 1 mRNAs expression after enterectomy

被引:24
作者
Avissar, NE [1 ]
Ziegler, TR
Wang, HT
Gu, LH
Miller, JNH
Iannoli, P
Leibach, FH
Ganapathy, V
Sax, HC
机构
[1] Univ Rochester, Med Ctr, Dept Surg, 601 Elmwood Ave, Rochester, NY 14642 USA
[2] Emory Univ, Sch Med, Dept Med, Atlanta, GA 30322 USA
[3] Med Coll Georgia, Dept Biochem & Mol Biol, Augsburg, Germany
关键词
D O I
10.1177/014860710102500265
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Background: Sucessful intestinal adaptation after massive enterectomy is dependent on increased efficiency of nutrient transport. However, midgut resection (MGR) in rabbits induces an initial decrease in sodium-dependent brush border neutral amino acid transport, whereas parenteral epidermal growth factor (EGF) and growth hormone (GH) reverse this downregulation. We investigated intestinal amino acid transporter B-0 (ATB(0)) and oligopeptide transporter 1 (PEPT 1) mRNA expression after resection and in response to EGF and/or GH. Methods: Rabbits underwent anesthesia alone (control) or proximal, midgut, and distal resections. Full-thickness intestine was harvested from all groups on postoperative day (POD) 7, and on POD 14 from control and MGR rabbits. A second group of MGR rabbits received EGF and/or GH for 7 days, beginning 7 days after resection. ATB(0) and PEPT I mRNA levels were determined by Northern blot analysis. Results: In control animals, ileal ATB(0) mRNA abundance was three times higher than jejunal mRNA, whereas PEPT I mRNA expression was similar. By 7 and 14 days after MGR, jejunal ATB(0) mRNA abundance was decreased by 50% vs control jejunum. A 50% decrease in jejunal PEPT 1 message was delayed until 14 days after MGR. Treatment with EGF plus GH did not alter ATB(0) mRNA expression but doubled PEPT 1 mRNA in the jejunum. Conclusion: The site of resection, time postresection, and growth factors treatment differentially influence ATB(0) and PEPT 1 mRNA expression. Enhanced sodium dependent brush border neutral amino acid transport with GH plus EGF administration is independent of increased ATB(0) mRNA expression in rabbit small intestine after enterectomy.
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收藏
页码:65 / 72
页数:8
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