A highly selective fluorescent probe for thiol bioimaging

被引：262

作者：

Bouffard, Jean ^{[1
,2
]}

Kim, Youngmi ^{[1
,2
]}

Swager, Timothy M. ^{[1
,2
]}

Weissleder, Ralph ^{[1
,2
]}

Hilderbrand, Scott A. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

[2] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

ORGANIC LETTERS | 2008年 / 10卷 / 01期

关键词：

D O I：

10.1021/ol702539v

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A new fluorescent turn-on probe (3) for the selective sensing and bioimaging of thiols is reported. In aqueous buffer solutions at physiological pH, thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the red-emissive donor-acceptor fluorophore (4). The probe displays excellent immunity to interference from nitrogen and oxygen nucleophiles and the imaging of thiols in living cells is demonstrated.

引用

页码：37 / 40

页数：4

共 30 条

[1]

BRAND VT, 1953, ANAL CHEM, V25, P769

[2] TISSUE SULFHYDRYL GROUPS [J].