Out-of-equilibrium pH transients in the guinea-pig ventricular myocyte

1. Following an intracellular alkali load (imposed by acetate prepulsing in CO2/HCO3- buffer), intracellular pH (pH(1)) of the guinea-pig ventricular myocyte (recorded from intracellular SNARF fluorescence) recovers to control levels. Recovery has two phases. An initial rapid phase (lasting up to 2 min) is followed by a later slow phase (several minutes). Inhibition of sarcolemmal acid-loading carriers (by removal of extracellular Cl-) inhibits the later, slow phase but the initial rapid recovery phase persists. It also persists in the absence of extracellular Na+ and in the presence of the HCO3- transport inhibitor DIDS (4,4-diisothiocyanatostilbene-2,2-disulphonic acid). 2. The rapid recovery phase is not evident if the alkali load has been induced by reducing P-CO2, (from 10 to 5%), and it is inhibited in the absence of CO2/HCO3- buffer (i.e. Hepes buffer). It is also slowed by the carbonic anhydrase (CA) inhibitor acetazolamide (ATZ). We conclude that it is caused by buffering of the alkali load through the hydration of intracellular CO2 (CO2-dependent buffering). 3. The time course of rapid recovery is consistent with an intracellular CO2 hydration rate constant (k(1)) of 0.36 s(-1) in the presence of CA activity, and 0.14 s(-1) in the absence of CA activity. This latter k(1) value matches the literature value for uncatalysed CO2 hydration in free solution. Natural CO2 hydration is accelerated 2.6-fold in the ventricular myocyte by endogenous CA. 4. The rapid recovery phase represents a period when the intracellular CO2/HCO3- buffer is out of equilibrium (OOE). Modelling of the recovery phase using our k(1) value, indicates that OOE conditions will normally extend for at least 2 min following a step rise in pH(i) (at constant P-CO2). If CA is inactive, this period can be as long as 5 min. During normal pH, regulation, the recovery rate during these periods cannot be used as a measure of sarcolemmal acid loading since it is a mixture of slow CO2-dependent buffering and transmembrane acid loading. The implication of this finding for quantification of pH, regulation during alkalosis is discussed.