Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth

被引：23

作者：

Arikawa, Y

Enomoto, K

Muratsubaki, H

Okazaki, M

机构：

[1] Food Technol Res Inst Nagano Prefecture, Nagano 3800921, Japan

[2] Kyorin Univ, Sch Hlth Sci, Dept Clin Biochem, Tokyo 1920005, Japan

[3] Shinshu Univ, Ctr Gene Res, Ueda, Nagano 3860018, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 165卷 / 01期

关键词：

fumarate reductase; OSM1; gene; promitochondrial enzyme; yeast;

D O I：

10.1016/S0378-1097(98)00260-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In Saccharomyces, cerevisiae, the cytosolic and promitochondrial isoenzymes of fumarate reductase are encoded by the FRDS and OSM1 genes, respectively. The product of the OSM1 gene is reported to be required for growth in hypertonic medium. Simultaneous disruption of the FRDS and OSM1 genes resulted in the inability of the yeasts to grow anaerobically on glucose as a carbon source, and disruption of the OSM1 gene caused poor growth under anaerobic conditions. However, the disruption of both the FRDS and/or OSM1 genes had no effect on aerobic growth or growth under hypertonic conditions. These results suggest that the fumarate reductase isoenzymes in Saccharomyces cerevisiae are essential for anaerobic growth but not for growth under hypertonic conditions. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：111 / 116

页数：6

共 19 条

[1] The two isoenzymes for yeast NAD(+)-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation [J].