Polyethylene glycol monomethyl ether sulfate-based background electrolytes in capillary electrophoresis

被引：12

作者：

Williams, RL ^{[1
]}

Vigh, G ^{[1
]}

机构：

[1] TEXAS A&M UNIV,DEPT CHEM,COLLEGE STN,TX 77843

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 744卷 / 1-2期

关键词：

background electrolyte composition; (polyethylene glycol monomethyl ether) hydrogensulfates; aminocaproic acid; dinitrobenzamidophenylalanine; nitrobenzoic acids;

D O I：

10.1016/0021-9673(96)00350-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A homologous series of (polyethylene glycol monomethyl ether) hydrogensulfates has been synthesized, characterized and used as the source of anionic co-ions in mobility-matching background electrolytes designed to eliminate the efficiency-degrading effects of electromigration dispersion. The mobilities of the new anionic co-ions vary in the 5 . 10(-5) to 45 . 10(-5) cm(2)/Vs range in 25 mM mobility-matching background electrolytes, prepared from epsilon-aminocaproic acid and the respective (polyethylene glycol monomethyl ether) hydrogensulfate. It is demonstrated experimentally that the electromigration dispersion-related peak broadening can be minimized and difficult electrophoretic separations can be realized without significant alteration of the separation selectivity when these (polyethylene glycol monomethyl ether) sulfate anions are used as co-ions in mobility-matching background electrolytes.

引用

页码：75 / 80

页数：6

共 14 条

[1] EXPERIMENTAL-EVIDENCE FOR THE EXISTENCE OF DUOSELECTIVE (TYPE-III) ENANTIOMER SEPARATIONS IN THE CAPILLARY ELECTROPHORETIC ANALYSIS OF CHIRAL WEAK ACIDS [J].