The modulation of platelet adhesion and activation by chitosan through plasma and extracellular matrix proteins

被引：140

作者：

Lord, Megan S. ^{[1
]}

Cheng, Bill ^{[1
]}

McCarthy, Simon J. ^{[2
]}

Jung, MoonSun ^{[1
]}

Whitelock, John M. ^{[1
]}

机构：

[1] Univ New S Wales, Grad Sch Biomed Engn, Sydney, NSW 2052, Australia

[2] HemCon Med Technol Inc, Portland, OR USA

来源：

BIOMATERIALS | 2011年 / 32卷 / 28期

基金：

澳大利亚研究理事会;

关键词：

Chitosan; Platelets; Integrins; Protein adsorption; CELL-ADHESION; IIB-IIIA; INTEGRIN; CHITIN; MEMBRANE; COLLAGEN; RECEPTOR; FIBRONECTIN; MECHANISMS; INHIBITOR;

D O I：

10.1016/j.biomaterials.2011.05.062

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Chitosan has been shown to promote initial wound closure events to prevent blood loss. Platelet adhesion and activation are crucial early events in these processes after traumatic bleeding leading to thrombus formation. Platelet adhesion to chitosan was found to be enhanced in the presence of adsorbed plasma and extracellular matrix proteins and was found to be primarily mediated by alpha(IIb)beta(3) integrins, while alpha(2)beta(1) integrins were found to be involved in platelet adhesion to collagen and perlecan. Platelets were found to be activated by chitosan, as shown by an increase in the expression of alpha(IIb)beta(3) integrins and P-selectin, while the extent of activation was modulated by the presence of proteins including perlecan and fibrinogen. Collagen-coated chitosan was found to activate platelets to the same extent as either chitosan or collagen alone. These data support the role of plasma and extracellular matrix proteins in promoting chitosan mediated platelet adhesion and activation supporting the hypothesis that chitosan promotes wound healing via these interactions. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：6655 / 6662

页数：8

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