Biotransformation of atrazine in transgenic tobacco cell culture expressing human P450

被引：26

作者：

Bode, M ^{[1
]}

Stöbe, P ^{[1
]}

Thiede, B ^{[1
]}

Schuphan, I ^{[1
]}

Schmidt, B ^{[1
]}

机构：

[1] Univ Aachen, Dept Biol 5, D-52056 Aachen, Germany

来源：

PEST MANAGEMENT SCIENCE | 2004年 / 60卷 / 01期

关键词：

human CYP1A1 and CYP1A2; transgenic plant cell culture; (Agrobacterium); atrazine; biotransformation;

D O I：

10.1002/ps.770

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Plant cell cultures in which the appropriate P450 cDNA is introduced are expected to metabolise certain pesticides in large quantities. Two species of human P450 (CYP1A1 and CYP1A2) were introduced into tobacco cells (Nicotiana tabacum L) by Agrobacterium-mediated transformation. The transgenic plant cell cultures were selected by combination of kanamycin-resistance, 7-ethoxycoumarin 0-deethylase activity, PCR and Western blot analysis. For metabolism studies, C-14-labelled atrazine was used as a model substance. The metabolites de-ethylatrazine and de-isopropylatrazine were found in the control culture as well as in the transgenic culture, whereas the non-phytotoxic metabolite de-ethyl-deisopropylatrazine was found only in the transgenic cell cultures. The results showed that both foreign enzymes CYP1A1 and CYP1A2 catalyse N-dealkylation of atrazine. However, CYPIA2 exhibited a higher conversion rate than CYP1A1. In a time-course study the enzyme CYPIA2 catalysed predominantly N-de-ethylation followed by de-isopropylation. The extent of metabolism was considerably higher than in non-transformed cell cultures. The transgenic cell cultures can therefore be suitable tools for the production of large quantities of primary oxidised pesticide metabolites. (C) 2003 Society of Chemical Industry

引用

页码：49 / 58

页数：10

共 27 条

[1] HIGH-EFFICIENCY TRANSFORMATION OF CULTURED TOBACCO CELLS [J].