Direct electrochemistry and electrocatalysis of hemoglobin immobilized in TiO2 nanotube films

被引：89

作者：

Zheng, W. ^{[1
,2
,3
]}

Zheng, Y. F. ^{[1
,2
]}

Jin, K. W. ^{[4
]}

Wang, N. ^{[4
]}

机构：

[1] Peking Univ, Coll Engn, LTCS, Beijing 100871, Peoples R China

[2] Peking Univ, Coll Engn, Dept Adv Mat & Nanotechnol, Beijing 100871, Peoples R China

[3] Harbin Engn Univ, Ctr Biomed Mat & Engn, Harbin 150001, Peoples R China

[4] Hong Kong Univ Sci & Technol, Dept Phys, Hong Kong, Hong Kong, Peoples R China

来源：

TALANTA | 2008年 / 74卷 / 05期

关键词：

titanium oxide nanotubes; hemoglobin; electrochemistry; hydrogen peroxide; biosensors;

D O I：

10.1016/j.talanta.2007.09.017

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Titanium oxide nanotubes (TiO2-NTs) synthesized by the hydrothermal method had been prepared as the co-immobilization matrix to incorporate hemoglobin (Hb) successfully. The nanostructures of TiO2-NTs were investigated by X-ray diffraction and high-resolution electron microscopy. The Hb immobilized in TiO2-NTs had a similar structure to the native of Hb and retained its near-native conformations as characterized by the UV-vis and FT-IR spectroscopy. A couple of quasi-reversible redox peaks with a formal potential of -0.34 V (vs. SCE) in 0.10 M pH 7.0 phosphate buffered saline (PBS) were observed. The amperometric response of the immobilized Hb linearly to H2O2 concentration ranged from 4 mu M to 64 mu M with a detection limit of 4.637 x 10(-6) M and the high stability of the immobilized Hb in TiO2-NTs constituted a promising platform for the development of biosensors. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1414 / 1419

页数：6