Crimean-Congo hemorrhagic fever virus glycoprotein processing by the endoprotease SKI-1/S1P is critical for virus infectivity

被引：80

作者：

Bergeron, Eric ^{[1
]}

Vincent, Martin J. ^{[1
]}

Nichol, Stuart T. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Special Pathogens Branch, Div Viral & Rickettsial Dis, Natl Ctr Zoonot Vector Borne & Enter Dis, Atlanta, GA 30329 USA

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 23期

关键词：

D O I：

10.1128/JVI.01647-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Crimean-Congo hemorrhagic fever virus (CCHFV) causes severe human disease. The CCHFV medium RNA encodes a polyprotein which is proteolytically processed to yield the glycoprotein precursors PreGn and PreGc, followed by structural glycoproteins Gn and Gc. Subtilisin kexin isozyme-1/site-1 protease (SKI-1/SIP) plays a central role in Gn processing. Here we show that CCHFV-infected cells deficient in SKI-1/SIP produce no infectious virus, although PreGn and PreGc accumulated normally in the Golgi apparatus, the site of virus assembly. Only nucleoprotein-containing particles which lacked virus glycoproteins (Gn/Ge or PreGn/PreGc) were secreted. Complementation of SKI-1/SlP-deficient cells with a SKI-1/SIP expression vector restored release of infectious virus (> 10(6) PFU/ml), confirming that SKI-1/SIP processing is required for incorporation of viral glycoproteins. SKI-1/SIP may represent a promising antiviral target.

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收藏

页码：13271 / 13276

页数：6

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