ChAM, a novel motif that mediates PALB2 intrinsic chromatin binding and facilitates DNA repair

被引：43

作者：

Bleuyard, Jean-Yves ^{[1
]}

Buisson, Remi ^{[2
]}

Masson, Jean-Yves ^{[2
]}

Esashi, Fumiko ^{[1
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[2] Univ Laval, Canc Res Ctr, Genome Stabil Lab, Hotel Dieu Quebec, Quebec City, PQ G1R 2J6, Canada

来源：

EMBO REPORTS | 2012年 / 13卷 / 02期

关键词：

PALB2; chromatin association; homologous recombination; DNA repair; cancer; HOMOLOGOUS RECOMBINATION; FANCONI-ANEMIA; CANCER; BRCA2; MUTATIONS; PARTNER; BREAKS; MRG15;

D O I：

10.1038/embor.2011.243

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The partner and localizer of breast cancer 2 susceptibility protein (PALB2) is crucial for the repair of DNA damage by homologous recombination. Here, we report that chromatin-association motif (ChAM), an evolutionarily conserved motif in PALB2, is necessary and sufficient to mediate its chromatin association in both unperturbed and damaged cells. ChAM is distinct from the previously described PALB2 DNA-binding regions. Deletion of ChAM decreases PALB2 and Rad51 accumulation at DNA damage sites and confers cellular hypersensitivity to the genotoxic drug mitomycin C. These results suggest that PALB2 chromatin association via ChAM facilitates PALB2 function in the cellular resistance to DNA damage.

引用

页码：135 / 141

页数：7

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