Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles

被引：395

作者：

Li, P

Li, J

Wu, CZ

Wu, QS ^{[1
]}

Li, J

机构：

[1] Tongji Univ, Dept Chem, Shanghai 200092, Peoples R China

[2] Anhui Agr Univ, Dept Food Sci & Technol, Hefei 200036, Peoples R China

来源：

NANOTECHNOLOGY | 2005年 / 16卷 / 09期

关键词：

D O I：

10.1088/0957-4484/16/9/082

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The bactericidal action of silver (0) nanoparticles and amoxicillin on Escherichia coli is studied, respectively. Increasing concentration of both arnoxicillin (0-0.525 mg ml(-1)) and silver nanoparticles (0-40 mu g ml(-1)) showed a higher antibacterial effect in Luria-Bertani (LB) medium. Escherichia coli cells have different bactericidal sensitivity to them. When amoxicillin and silver nanoparticles are combined, it results in greater bactericidal efficiency on Escherichia coli cells than when they were applied separately. Dynamic tests on bacterial growth indicated that exponential and stationary phases are greatly decreased and delayed in the synergistic effect of amoxicillin and silver nanoparticles. In addition, the effect induced by a preincubation with silver nanoparticles is examined. The results show that solutions with more silver nanoparticles have better antimicrobial effects. One hypothesized mechanism is proposed to explain this phenomenon.

引用

收藏

页码：1912 / 1917

页数：6

相关论文

共 31 条

[21] THE CRISIS IN ANTIBIOTIC-RESISTANCE [J].

NEU, HC .

SCIENCE, 1992, 257 (5073) :1064-1073

[22] Molecular epidemiology of Pseudomonas aeruginosa colonization in a burn unit:: Persistence of a multidrug-resistant clone and a silver sulfadiazine-resistant clone [J].

Pirnay, JP ;

De Vos, D ;

Cochez, C ;

Bilocq, F ;

Pirson, J ;

Struelens, M ;

Duinslaeger, L ;

Cornelis, P ;

Zizi, M ;

Vanderkelen, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (03) :1192-1202

[23] Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli [J].

Pitout, JDD ;

Sanders, CC ;

Sanders, WE .

AMERICAN JOURNAL OF MEDICINE, 1997, 103 (01) :51-59

[24]

ROBERT FS, 2004, SCIENCE, V303, P1789

[25] Surface-grafted viologen for precipitation of silver nanoparticles and their combined bactericidal activities [J].

Shi, ZL ;

Neoh, KG ;

Kang, ET .

LANGMUIR, 2004, 20 (16) :6847-6852

[26] Silver nanoparticles as antimicrobial agent:: a case study on E-coli as a model for Gram-negative bacteria [J].

Sondi, I ;

Salopek-Sondi, B .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2004, 275 (01) :177-182

[27] In vitro activity of BAY 12-8039, a new fluoroquinolone, against species representative of respiratory tract pathogens [J].

Souli, M ;

Wennersten, CB ;

Eliopoulos, GM .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 1998, 10 (01) :23-30

[28] The use of amoxicillin and ticarcillin in combination with a beta-lactamase inhibitor as decontaminating agents in the Agrobacterium tumefaciens-mediated transformation of Artemisia annua L [J].

Vergauwe, A ;

VanGeldre, E ;

Inze, D ;

VanMontagu, M ;

vandenEeckhout, E .

JOURNAL OF BIOTECHNOLOGY, 1996, 52 (02) :89-95

[29] ROLE OF BETA-LACTAM HYDROLYSIS IN THE MECHANISM OF RESISTANCE OF A BETA-LACTAMASE-CONSTITUTIVE ENTEROBACTER-CLOACAE STRAIN TO EXPANDED-SPECTRUM BETA-LACTAMS [J].

VU, H ;

NIKAIDO, H .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1985, 27 (03) :393-398

[30] Synthesis and antibacterial activity of dual-action agents of a β-lactam antibiotic with cytotoxic agent mitozolomide or temozolomide [J].

Wang, YF ;

Lambert, P ;

Zhao, LX ;

Wang, D .

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2002, 37 (04) :323-332

← 1 2 3 4 →