A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M)

被引：160

作者：

Leeming, D. J. ^{[1
,2
]}

He, Y. ^{[3
]}

Veidal, S. S. ^{[1
]}

Nguyen, Q. H. T. ^{[1
]}

Larsen, D. V. ^{[1
]}

Koizumi, M. ^{[4
]}

Segovia-Silvestre, T. ^{[1
]}

Zhang, C. ^{[1
]}

Zheng, Q. ^{[3
]}

Sun, S. ^{[3
]}

Cao, Y. ^{[3
]}

Barkholt, V. ^{[2
]}

Hagglund, P. ^{[2
]}

Bay-Jensen, A. C. ^{[5
]}

Qvist, P. ^{[5
]}

Karsdal, M. A. ^{[1
]}

机构：

[1] Nord Biosci, Fibrosis Biol & Biomarkers, Herlev, Denmark

[2] Tech Univ Denmark, Dept Syst Biol, DK-2800 Lyngby, Denmark

[3] Nord Biosci Beijing, Beijing, Peoples R China

[4] Natl Inst Radiol Sci, Chiba 260, Japan

[5] Nord Biosci, Cartilage Biol & Biomarkers, Herlev, Denmark

来源：

BIOMARKERS | 2011年 / 16卷 / 07期

关键词：

Biochemical markers; type I collagen; liver fibrosis; protease-cleaved neo-epitope; MMP-2; -9; -13; translational science; bile duct ligation; CCL4; rat model; breast cancer; prostate cancer; bone metastases; CONNECTIVE-TISSUE; SAMPLING VARIABILITY; SERUM CONCENTRATION; FIBROSIS; TELOPEPTIDE; ICTP; DEGRADATION; EXPRESSION; BIOMARKERS; PROSTATE;

D O I：

10.3109/1354750X.2011.620628

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

A competitive enzyme-linked immunosorbent assay (ELISA) for detection of a type I collagen fragment generated by matrix metalloproteinases (MMP) -2, -9 and -13, was developed (CO1-764 or C1M). The biomarker was evaluated in two preclinical rat models of liver fibrosis: bile duct ligation (BDL) and carbon tetra chloride (CCL4)-treated rats. The assay was further evaluated in a clinical study of prostate-, lung-and breast-cancer patients stratified according to skeletal metastases. A technically robust ELISA assay specific for a MMP-2, -9 and -13 neo-epitope was produced and seen to be statistically elevated in BDL rats compared to baseline levels as well as significantly elevated in CCL4 rats stratified according to the amount of total collagen in the livers. CO1-764 levels also correlated significantly with total liver collagen and type I collagen mRNA expression in the livers. Finally, the CO1-764 marker was not correlated with skeletal involvement or number of bone metastases. This ELISA has the potential to assess the degree of liver fibrosis in a non-invasive manner.

引用

页码：616 / 628

页数：13

共 48 条

[1]

A novel assay for extracellular matrix remodeling associated with liver fibrosis: An enzyme-linked immunosorbent assay (ELISA) for a MMP-9 proteolytically revealed neo-epitope of type III collagen [J].

Barascuk, N. ;

Veidal, S. S. ;

Larsen, L. ;

Larsen, D. V. ;

Larsen, M. R. ;

Wang, J. ;

Zheng, Q. ;

Xing, R. ;

Cao, Y. ;

Rasmussen, L. M. ;

Karsdal, M. A. .

CLINICAL BIOCHEMISTRY, 2010, 43 (10-11) :899-904

[2]

Sampling variability of liver fibrosis in chronic hepatitis C [J].

Bedossa, P ;

Dargère, D ;

Paradis, V .

HEPATOLOGY, 2003, 38 (06) :1449-1457

[3]

Clària J, 1999, ASCITES AND RENAL DYSFUNCTION IN LIVER DISEASE, P379

[4]

Clinical features of metastatic bone disease and risk of skeletal morbidity [J].

Coleman, Robert E. .

CLINICAL CANCER RESEARCH, 2006, 12 (20) :6243S-6249S

[5]

NPS@:: Network Protein Sequence Analysis [J].

Combet, C ;

Blanchet, C ;

Geourjon, C ;

Deléage, G .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (03) :147-150

[6]

The biochemistry of metastasis [J].

Duffy, MJ .

ADVANCES IN CLINICAL CHEMISTRY, VOL 32, 1996, 32 :135-166

[7]

SERUM CONCENTRATION OF THE CROSS-LINKED CARBOXYTERMINAL TELOPEPTIDE OF TYPE-I COLLAGEN (ICTP) IS A USEFUL PROGNOSTIC INDICATOR IN MULTIPLE-MYELOMA [J].

ELOMAA, I ;

VIRKKUNEN, P ;

RISTELI, L ;

RISTELI, J .

BRITISH JOURNAL OF CANCER, 1992, 66 (02) :337-341

[8]

FREIJE JMP, 1994, J BIOL CHEM, V269, P16766

[9]

Liver fibrosis - from bench to bedside [J].

Friedman, SL .

JOURNAL OF HEPATOLOGY, 2003, 38 :S38-S53

[10]

The type I collagen fragments ICTP and CTX reveal distinct enzymatic pathways of bone collagen degradation [J].

Garnero, P ;

Ferreras, M ;

Karsdal, MA ;

Nicamhlaoibh, R ;

Risteli, J ;

Borel, O ;

Qvist, P ;

Delmas, PD ;

Foged, NT ;

Delaissé, JM .

JOURNAL OF BONE AND MINERAL RESEARCH, 2003, 18 (05) :859-867

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