A novel assay for extracellular matrix remodeling associated with liver fibrosis: An enzyme-linked immunosorbent assay (ELISA) for a MMP-9 proteolytically revealed neo-epitope of type III collagen

被引：196

作者：

Barascuk, N. ^{[1
,3
]}

Veidal, S. S. ^{[1
,4
]}

Larsen, L. ^{[1
]}

Larsen, D. V. ^{[1
]}

Larsen, M. R. ^{[5
]}

Wang, J. ^{[2
]}

Zheng, Q. ^{[2
]}

Xing, R. ^{[2
]}

Cao, Y. ^{[2
]}

Rasmussen, L. M. ^{[3
]}

Karsdal, M. A. ^{[1
,3
]}

机构：

[1] Nord Biosci AS, Herlev, Denmark

[2] Nord Biosci Beijing, Beijing, Peoples R China

[3] Univ So Denmark, Dept Biochem Pharmacol & Genet, Odense Univ Hosp, Odense M, Denmark

[4] Univ So Denmark, Inst Clin, Odense M, Denmark

[5] Univ So Denmark, Inst Biochem & Mol Biol, Odense M, Denmark

来源：

CLINICAL BIOCHEMISTRY | 2010年 / 43卷 / 10-11期

关键词：

Extracellular matrix remodelling; Type III collagen; Matrix-metallo-proteinase-9; Liver fibrosis; ELISA; CARTILAGE DEGRADATION; ARTICULAR-CARTILAGE; THERAPEUTIC TARGETS; MOUSE MODELS; BIOMARKERS; OSTEOARTHRITIS; METALLOPROTEINASES; CELLS; BONE;

D O I：

10.1016/j.clinbiochem.2010.03.012

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

100118 [医学信息学]; 100208 [临床检验诊断学];

摘要：

Objectives: Accumulation of extracellular matrix (ECM) components and increased matrix-metalloprotease (MMPs) activity are hallmarks of fibrosis. We developed an ELISA for quantification of MMP-9 derived collagen type III (CO3) degradation. Design and methods: A monoclonal antibody targeting a specific MMP-9 cleaved fragment of CO3 was used for development of a competitive ELISA. The assay was investigated in serum and tissues from bile duct ligated rats (BDL). Results: The ELISA showed no cross-reaction with either intact CO3, or other collagens. The intra- and inter-assay CV were below 10%. Liver fibrosis was demonstrated in BDL animals by semi quantitative scoring (P<0.0001). Serum levels of CO3-610 increased 2.5 fold in BDL animals (P<0.001). The CO3-610 levels were 5 fold higher in ex vivo cultures of fibrotic livers compared to controls (P<0.001). Conclusion: We have developed a novel ELISA for measuring a specific fragment CO3 generated by MMP-9 important in pathogenesis of liver fibrosis. (c) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：899 / 904

页数：6

共 29 条

[1]

Oral salmon calcitonin induced suppression of urinary collagen type II degradation in postmenopausal women:: A new potential treatment of osteoarthritis [J].

Bagger, YZ ;

Tankó, LB ;

Alexandersen, P ;

Karsdal, MA ;

Olson, M ;

Mindeholm, L ;

Azria, M ;

Christiansen, C .

BONE, 2005, 37 (03) :425-430

[2]

Liver fibrosis [J].

Bataller, R ;

Brenner, DA .

JOURNAL OF CLINICAL INVESTIGATION, 2005, 115 (02) :209-218

[3]

Classification of osteoarthritis biomarkers:: a proposed approach [J].

Bauer, D. C. ;

Hunter, D. J. ;

Abramson, S. B. ;

Attur, M. ;

Corr, M. ;

Felson, D. ;

Heinegard, D. ;

Jordan, J. M. ;

Kepler, T. B. ;

Lane, N. E. ;

Saxne, T. ;

Tyree, B. ;

Kraus, V. B. .

OSTEOARTHRITIS AND CARTILAGE, 2006, 14 (08) :723-727

[4]

BOTNEY MD, 1992, AM J PATHOL, V140, P357

[5]

Reducing risks to health, promoting healthy life [J].

Brundtland, GH .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2002, 288 (16) :1974-1974

[6]

Liver fibrosis - from bench to bedside [J].

Friedman, SL .

JOURNAL OF HEPATOLOGY, 2003, 38 :S38-S53

[7]

Matrix metalloproteinases in vascular remodeling and atherogenesis - The good, the bad, and the ugly [J].

Galis, ZS ;

Khatri, JJ .

CIRCULATION RESEARCH, 2002, 90 (03) :251-262

[8]

The type I collagen fragments ICTP and CTX reveal distinct enzymatic pathways of bone collagen degradation [J].

Garnero, P ;

Ferreras, M ;

Karsdal, MA ;

Nicamhlaoibh, R ;

Risteli, J ;

Borel, O ;

Qvist, P ;

Delmas, PD ;

Foged, NT ;

Delaissé, JM .

JOURNAL OF BONE AND MINERAL RESEARCH, 2003, 18 (05) :859-867

[9]

SIMPLE METHOD FOR POLYETHYLENE GLYCOL-PROMOTED HYBRIDIZATION OF MOUSE MYELOMA CELLS [J].

GEFTER, ML ;

MARGULIES, DH ;

SCHARFF, MD .

SOMATIC CELL GENETICS, 1977, 3 (02) :231-236

[10]

Collagens -: structure, function, and biosynthesis [J].

Gelse, K ;

Pöschl, E ;

Aigner, T .

ADVANCED DRUG DELIVERY REVIEWS, 2003, 55 (12) :1531-1546

← 1 2 3 →