A miniature head-mounted two-photon microscope: High-resolution brain imaging in freely moving animals

被引:488
作者
Helmchen, F
Fee, MS
Tank, DW
Denk, W [1 ]
机构
[1] Lucent Technol, Bell Labs, Biol Computat Res Dept, Murray Hill, NJ 07974 USA
[2] Max Planck Inst Med Res, Dept Biomed Opt, D-69120 Heidelberg, Germany
关键词
D O I
10.1016/S0896-6273(01)00421-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Two-photon microscopy has enabled anatomical and functional fluorescence imaging in the intact brain of rats. Here, we extend two-photon imaging from anesthetized, head-stabilized to awake, freely moving animals by using a miniaturized head-mounted microscope. Excitation light is conducted to the microscope in a single-mode optical fiber, and images are scanned using vibrations of the fiber tip. Microscope performance was first characterized in the neocortex of anesthetized rats. We readily obtained images of vasculature filled with fluorescently labeled blood and of layer 2/3 pyramidal neurons filled with a calcium indicator. Capillary blood flow and dendritic calcium transients were measured with high time resolution using line scans. In awake, freely moving rats, stable imaging was possible except during sudden head movements.
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页码:903 / 912
页数:10
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