A miniature head-mounted two-photon microscope: High-resolution brain imaging in freely moving animals
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作者:
Helmchen, F
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机构:Lucent Technol, Bell Labs, Biol Computat Res Dept, Murray Hill, NJ 07974 USA
Helmchen, F
Fee, MS
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机构:Lucent Technol, Bell Labs, Biol Computat Res Dept, Murray Hill, NJ 07974 USA
Fee, MS
Tank, DW
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机构:Lucent Technol, Bell Labs, Biol Computat Res Dept, Murray Hill, NJ 07974 USA
Tank, DW
Denk, W
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Lucent Technol, Bell Labs, Biol Computat Res Dept, Murray Hill, NJ 07974 USALucent Technol, Bell Labs, Biol Computat Res Dept, Murray Hill, NJ 07974 USA
Denk, W
[1
]
机构:
[1] Lucent Technol, Bell Labs, Biol Computat Res Dept, Murray Hill, NJ 07974 USA
[2] Max Planck Inst Med Res, Dept Biomed Opt, D-69120 Heidelberg, Germany
Two-photon microscopy has enabled anatomical and functional fluorescence imaging in the intact brain of rats. Here, we extend two-photon imaging from anesthetized, head-stabilized to awake, freely moving animals by using a miniaturized head-mounted microscope. Excitation light is conducted to the microscope in a single-mode optical fiber, and images are scanned using vibrations of the fiber tip. Microscope performance was first characterized in the neocortex of anesthetized rats. We readily obtained images of vasculature filled with fluorescently labeled blood and of layer 2/3 pyramidal neurons filled with a calcium indicator. Capillary blood flow and dendritic calcium transients were measured with high time resolution using line scans. In awake, freely moving rats, stable imaging was possible except during sudden head movements.
机构:
Warner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USAWarner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USA
Davis, MD
Schmidt, JJ
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机构:
Warner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USAWarner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USA
机构:
Warner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USAWarner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USA
Davis, MD
Schmidt, JJ
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机构:
Warner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USAWarner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Neurosci Therapeut, Ann Arbor, MI 48105 USA