Detection of conformation bias between the complementary strands of deoxynucleotide repeat (TG/AC)(n)

被引：6

作者：

Dobi, A

Mahan, MA

VonAgoston, D

机构：

[1] NIH,NICHD,LDN,UNIT MOL CONTROL NEURODIFFERENTIAT,BETHESDA,MD 20892

[2] PRINCETON UNIV,UNDERGRAD PROGRAM,PRINCETON,NJ 08544

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 01期

关键词：

DNA-repeat; nucleic acid conformation; polyacrylamide gel electrophoresis; DNA;

D O I：

10.1002/elps.1150180104

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antiparallel complementary strands of DNA contribute symmetrically to the helical structure and its stability; rough conformational differences between complementary strands would interfere with this symmetric contribution and the stability. To detect conformational differences between complementary strands, we challenged the complementary strands to different ''pore size'' of acrylamide gels and measured differences in migration during electrophoretic mobility analysis. Changes in migration differences revealed a significant structural bias between the complementary strands of the dinucleotide repeat deoxy-(TG/AC)(n).

引用

页码：12 / 16

页数：5

共 32 条

[1] CLUES TO THE PATHOGENESIS OF FAMILIAL COLORECTAL-CANCER
AALTONEN, LA
PELTOMAKI, P
LEACH, FS
SISTONEN, P
PYLKKANEN, L
MECKLIN, JP
JARVINEN, H
POWELL, SM
JEN, J
HAMILTON, SR
PETERSEN, GM
KINZLER, KW
VOGELSTEIN, B
DELACHAPELLE, A
[J]. SCIENCE, 1993, 260 (5109) : 812 - 816
[2] CALLEN DF, 1993, AM J HUM GENET, V52, P922
[3] PARALLEL DNA TRIPLEXES, HOMOLOGOUS RECOMBINATION, AND OTHER HOMOLOGY-DEPENDENT DNA INTERACTIONS
CAMERINIOTERO, RD
HSIEH, P
[J]. CELL, 1993, 73 (02) : 217 - 223
[4] GENETIC-LINKAGE BETWEEN VONHIPPEL-LINDAU DISEASE AND 3 MICROSATELLITE POLYMORPHISMS REFINES THE LOCALIZATION OF THE VHL LOCUS
CROSSEY, PA
MAHER, ER
JONES, MH
RICHARDS, FM
LATIF, F
PHIPPS, ME
LUSH, M
FOSTER, K
TORY, K
GREEN, JS
OOSTRA, B
YATES, JRW
LINEHAN, WM
AFFARA, NA
LERMAN, M
ZBAR, B
NAKAMURA, Y
FERGUSONSMITH, MA
[J]. HUMAN MOLECULAR GENETICS, 1993, 2 (03) : 279 - 282
[5] DOBI A L, 1990, Technique (Philadelphia), V2, P167
[6] GUANINE SPECIFIC CHEMICAL SEQUENCING OF DNA BY OSMIUM-TETROXIDE
DOBI, AL
MATSUMOTO, K
SANTHA, E
VONAGOSTON, D
[J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (22) : 4846 - 4847
[7] POLYNUCLEOTIDES .18. SINGLE-STRANDED NUCLEIC-ACID HELICAL SECONDARY STRUCTURE STABILIZED BY IONIC BONDS - D(A+-G)10
DOLINNAYA, NG
FRESCO, JR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) : 9242 - 9246
[8] PRIMARY SEQUENCE OF -1436 TO +53 BP OF THE RAT PREPROENKEPHALIN GENE PUTATIVE Z-DNA AND REGULATORY MOTIFS
DURKIN, RC
WEISINGER, G
HOLLOWAY, MP
LAGAMMA, EF
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1131 (03) : 349 - 351
[9] PRESYMPTOMATIC DIAGNOSIS IN FAMILIES WITH ADENOMATOUS POLYPOSIS USING HIGHLY POLYMORPHIC DINUCLEOTIDE CA REPEAT MARKERS FLANKING THE APC GENE
ECKERT, WA
JUNG, C
WOLFF, G
[J]. JOURNAL OF MEDICAL GENETICS, 1994, 31 (06) : 442 - 447
[10] DESCRIPTION OF A 2ND MICROSATELLITE MARKER AND LINKAGE ANALYSIS OF THE MUSCLE GLYCOGEN-SYNTHASE LOCUS IN FAMILIAL NIDDM
ELBEIN, SC
HOFFMAN, M
RIDINGER, D
OTTERUD, B
LEPPERT, M
[J]. DIABETES, 1994, 43 (08) : 1061 - 1065

← 1 2 3 4 →