A Broadly Neutralizing Human Monoclonal Antibody That Recognizes a Conserved, Novel Epitope on the Globular Head of the Influenza H1N1 Virus Hemagglutinin

被引：164

作者：

Krause, Jens C.

Tsibane, Tshidi ^{[3
]}

Tumpey, Terrence M. ^{[4
]}

Huffman, Chelsey J.

Basler, Christopher F. ^{[3
]}

Crowe, James E., Jr. ^{[1
,2
]}

机构：

[1] Vanderbilt Univ, Med Ctr, Vanderbilt Vaccine Ctr, Dept Pediat, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Med Ctr, Dept Microbiol & Immunol, Nashville, TN 37232 USA

[3] Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA

[4] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA 30333 USA

来源：

JOURNAL OF VIROLOGY | 2011年 / 85卷 / 20期

关键词：

B-CELLS; ORIGIN;

D O I：

10.1128/JVI.00700-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The conserved influenza virus hemagglutinin (HA) stem domain elicits cross-reactive antibodies, but epitopes in the globular head typically elicit strain-specific responses because of the hypervariability of this region. We isolated human monoclonal antibody 5J8, which neutralized a broad spectrum of 20th century H1N1 viruses and the 2009 pandemic H1N1 virus. Fine mapping of the interaction unexpectedly revealed a novel epitope between the receptor-binding pocket and the Ca(2) antigenic site on HA. This antibody exposes a new mechanism underlying broad immunity against H1N1 influenza viruses and identifies a conserved epitope that might be incorporated into engineered H1 virus vaccines.

引用

页码：10905 / 10908

页数：4

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