A Broadly Neutralizing Human Monoclonal Antibody That Recognizes a Conserved, Novel Epitope on the Globular Head of the Influenza H1N1 Virus Hemagglutinin

被引:164
作者
Krause, Jens C.
Tsibane, Tshidi [3 ]
Tumpey, Terrence M. [4 ]
Huffman, Chelsey J.
Basler, Christopher F. [3 ]
Crowe, James E., Jr. [1 ,2 ]
机构
[1] Vanderbilt Univ, Med Ctr, Vanderbilt Vaccine Ctr, Dept Pediat, Nashville, TN 37232 USA
[2] Vanderbilt Univ, Med Ctr, Dept Microbiol & Immunol, Nashville, TN 37232 USA
[3] Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA
[4] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA 30333 USA
关键词
B-CELLS; ORIGIN;
D O I
10.1128/JVI.00700-11
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The conserved influenza virus hemagglutinin (HA) stem domain elicits cross-reactive antibodies, but epitopes in the globular head typically elicit strain-specific responses because of the hypervariability of this region. We isolated human monoclonal antibody 5J8, which neutralized a broad spectrum of 20th century H1N1 viruses and the 2009 pandemic H1N1 virus. Fine mapping of the interaction unexpectedly revealed a novel epitope between the receptor-binding pocket and the Ca(2) antigenic site on HA. This antibody exposes a new mechanism underlying broad immunity against H1N1 influenza viruses and identifies a conserved epitope that might be incorporated into engineered H1 virus vaccines.
引用
收藏
页码:10905 / 10908
页数:4
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