Functional and possible physical association of scavenger receptor with cytoplasmic tyrosine kinase Lyn in monocytic THP-1-derived macrophages

被引：46

作者：

Miki, S

Tsukada, S

Nakamura, Y

Aimoto, S

Hojo, H

Sato, B

Yamamoto, M

Miki, Y

机构：

[1] MIKI CLIN,NEYAGAWA,OSAKA 572,JAPAN

[2] NISSEI HOSP,NISHI KU,OSAKA 550,JAPAN

[3] OSAKA UNIV,DEPT INTERNAL MED 3,SUITA,OSAKA 565,JAPAN

[4] OSAKA UNIV,DEPT NEUROPSYCHIAT,OSAKA 553,JAPAN

[5] OSAKA UNIV,INST PROT RES,OSAKA,JAPAN

[6] OSAKA CITY UNIV,FAC ENGN,DEPT BIOAPPL CHEM,OSAKA 558,JAPAN

来源：

FEBS LETTERS | 1996年 / 399卷 / 03期

关键词：

THP-1-derived macrophage; Lyn; scavenger receptor; acetyl low-density lipoprotein;

D O I：

10.1016/S0014-5793(96)01332-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetyl LDL (modified low-density lipoprotein), which is thought to be taken up through scavenger receptor A (SR-A), rapidly induced the appearance of phosphotyrosine proteins in monocytic THP-1-derived macrophages in vitro. The two alternative forms of Lyn (p(53) and p(56)) were found to be tyrosine-phosphorylated within 30 s after the stimulation with acetyl LDL, The catalytic activity of Lyn measured by an in vitro kinase assay had also increased in acetyl LDL-stimulated THP-1-derived macrophages, Furthermore, Lyn could be coimmunoprecipitated with SR-A from the cell lysate, These observations suggest a functional and possible physical association of SR-A with Lyn in THP-1-derived macrophages, and also imply a possible involvement of Lyn in SR-A signal transduction.

引用

页码：241 / 244

页数：4

共 21 条

[1] LOW-DENSITY-LIPOPROTEIN ACTIVATES MONOCYTES TO EXPRESS TUMOR-NECROSIS-FACTOR [J].