Separation and determination of podophyllum lignans by micellar electrokinetic chromatography

被引：39

作者：

Liu, SH ^{[1
]}

Tian, XA ^{[1
]}

Chen, XG ^{[1
]}

Hu, ZD ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 928卷 / 01期

关键词：

Podophyllum spp; plant materials; lignans;

D O I：

10.1016/S0021-9673(01)01096-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A micellar electrokinetic chromatography method was established for the quantitative analysis of seven podophyllum lignans in Podophyllum emodi Wall. var. chinesis sprague. The optimum buffer system was 10 mM NaH2PO4-5 mM borate- 100 mM sodium dodecylsulfate-30% isopropanol (pH 7.20). Voltage was 18 kV and detection at 214 nm. The second derivative chromatogram was used to determine a low-content component and those not fully separated from adjacent ones. The RSD values of migration times and peak areas were <2.2 and <5.5%, respectively. The effects of several CE parameters on the resolutions were studied systematically. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：109 / 115

页数：7

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