Systematic spatial analysis of gene expression during wheat caryopsis development

被引:89
作者
Drea, S
Leader, DJ
Arnold, BC
Shaw, P
Dolan, L
Doonan, JH [1 ]
机构
[1] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England
[2] Syngenta, Wheat Improvement Ctr, Norwich NR4 7UH, Norfolk, England
基金
英国生物技术与生命科学研究理事会; 英国自然环境研究理事会;
关键词
D O I
10.1105/tpc.105.034058
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cereal caryopsis is a complex tissue in which maternal and endosperm tissues follow distinct but coordinated developmental programs. Because of the hexaploid genome in wheat ( Triticum aestivum), the identification of genes involved in key developmental processes by genetic approaches has been difficult. To bypass this limitation, we surveyed 888 genes that are expressed during caryopsis development using a novel high- throughput mRNA in situ hybridization method. This survey revealed novel distinct spatial expression patterns that either reflected the ontogeny of the developing caryopsis or indicated specialized cellular functions. We have identified both known and novel genes whose expression is cell cycle - dependent. We have identified the crease region as important in setting up the developmental patterning, because the transition from proliferation to differentiation spreads from this region to the rest of the endosperm. A comparison of this set of genes with the rice ( Oryza sativa) genome shows that approximately two- thirds have rice counterparts but also suggests considerable divergence with regard to proteins involved in grain filling. We found that the wheat genes had significant homology with 350 Arabidopsis thaliana genes. At least 25 of these are already known to be essential for seed development in Arabidopsis, but many others remain to be characterized.
引用
收藏
页码:2172 / 2185
页数:14
相关论文
共 69 条
[1]   ESTs, cDNA microarrays, and gene expression profiling: tools for dissecting plant physiology and development [J].
Alba, R ;
Fei, ZJ ;
Payton, P ;
Liu, Y ;
Moore, SL ;
Debbie, P ;
Cohn, J ;
D'Ascenzo, M ;
Gordon, JS ;
Rose, JKC ;
Martin, G ;
Tanksley, SD ;
Bouzayen, M ;
Jahn, MM ;
Giovannoni, J .
PLANT JOURNAL, 2004, 39 (05) :697-714
[2]  
[Anonymous], 1962, ANATOMY SEED PLANTS
[3]   FTIR imaging of wheat endosperm cell walls in situ reveals compositional and architectural heterogeneity related to grain hardness [J].
Barron, C ;
Parker, ML ;
Mills, ENC ;
Rouau, X ;
Wilson, RH .
PLANTA, 2005, 220 (05) :667-677
[4]   Cell fate specification in the cereal endosperm [J].
Becraft, PW .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2001, 12 (05) :387-394
[5]   A gene expression map of the Arabidopsis root [J].
Birnbaum, K ;
Shasha, DE ;
Wang, JY ;
Jung, JW ;
Lambert, GM ;
Galbraith, DW ;
Benfey, PN .
SCIENCE, 2003, 302 (5652) :1956-1960
[6]   A structured mutant population for forward and reverse genetics in Barley (Hordeum vulgare L.) [J].
Caldwell, DG ;
McCallum, N ;
Shaw, P ;
Muehlbauer, GJ ;
Marshall, DF ;
Waugh, R .
PLANT JOURNAL, 2004, 40 (01) :143-150
[7]  
CEJUDO FJ, 1992, PLANT J, V2, P937
[8]   EB1 reveals mobile microtubule nucleation sites in Arabidopsis [J].
Chan, J ;
Calder, GM ;
Doonan, JH ;
Lloyd, CW .
NATURE CELL BIOLOGY, 2003, 5 (11) :967-971
[9]   Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration [J].
Chen, FQ ;
Foolad, MR .
PLANT MOLECULAR BIOLOGY, 1997, 35 (06) :821-831
[10]   A new resource for cereal genomics: 22K barley GeneChip comes of age [J].
Close, TJ ;
Wanamaker, SI ;
Caldo, RA ;
Turner, SM ;
Ashlock, DA ;
Dickerson, JA ;
Wing, RA ;
Muehlbauer, GJ ;
Kleinhofs, A ;
Wise, RP .
PLANT PHYSIOLOGY, 2004, 134 (03) :960-968