Future of liquid chromatography-mass spectrometry in metabolic profiling and metabolomic studies for biomarker discovery

被引：72

作者：

Metz, Thomas O. ^{[1
]}

Zhang, Qibin ^{[1
]}

Page, Jason S. ^{[1
]}

Shen, Yufeng ^{[1
]}

Callister, Stephen J. ^{[1
]}

Jacobs, Jon M. ^{[1
]}

Smith, Richard D. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Richland, WA 99352 USA

来源：

BIOMARKERS IN MEDICINE | 2007年 / 1卷 / 01期

关键词：

D O I：

10.2217/17520363.1.1.159

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The future utility of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discovery is discussed, beginning with a brief description of the evolution of metabolomics and the utilization of the three most popular analytical platforms in such studies: nuclear magnetic resonance, gas chromatography-MS and LC-MS. Emphasis is placed on recent developments in high-efficiency LC separations, sensitive electrospray ionization approaches and the benefits of incorporating both in LC-MS-based approaches. The advantages and disadvantages of various quantitative approaches are reviewed, followed by the current LC-MS-based tools available for candidate biomarker characterization and identification. Finally, a brief prediction on the future path of LC-MS-based methods in metabolic profiling and metabolomic studies is given.

引用

页码：159 / 185

页数：27

共 167 条

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