Metabolites from cerebrospinal fluid in aneurysmal subarachnoid haemorrhage correlate with vasospasm and clinical outcome:: a pattern-recognition 1H NMR study

被引:49
作者
Dunne, VG
Bhattachayya, S
Besser, M
Rae, C
Griffin, JL
机构
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[2] Royal Prince Alfred Hosp, Inst Clin Neurosci, Dept Neurosurg, Sydney, NSW, Australia
[3] Univ Sydney, Sch Mol & Microbial Biosci, Sydney, NSW, Australia
[4] Univ London Imperial Coll Sci Technol & Med, Dept Biol Chem, London, England
[5] Univ Sydney, Dept Surg, Sydney, NSW, Australia
关键词
cerebrospinal fluid; metabolomics; metabonomics; pattern recognition; principal components analysis; subarachnoid haemorrhage;
D O I
10.1002/nbm.918
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Following subarachnoid haemorrhage the most significant complication is sustained cerebral vascular contraction (vasospasm), which may result in terminal brain damage from cerebral infarction. Despite this, the biochemical cause of vasospasm remains poorly understood. In this study, the global high-concentration metabolite composition of CSF has been correlated with patient outcome after subarachnoid haemorrhage using multivariate statistics and H-1 NMR spectroscopy. In total, 16 patients with aneurysmal subarachnoid haemorrhage (aSAH) were compared with 16 control patients who required a procedure where CSF was obtained but did not have aSAH. Multivariate statistics readily distinguished the aSAH group from the heterogeneous control roup, even when only those controls with blood contamination in the CSF were used. Using principal components analysis and orthogonal signal correction, vasospasm was correlated to the concentrations of lactate, glucose and glutamine. These pattern recognition models of the NMR data also predicted Glasgow Coma Score (54% within +/-1 of the actual score on a scale of 1-15 for the whole patient group), Hunt and Hess SAH severity score (88% within +/-1 of the actual score on a scale of 1-5 for the aSAH group) and cognitive outcome scores (78% within +/-3 of the actual score on a 100% scale for the whole patient group). Thus, the approach allowed the prediction of outcome as well as confirming the presence of aSAH. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码:24 / 33
页数:10
相关论文
共 46 条
[1]   EVALUATION OF CEREBROVASCULAR SPASM WITH TRANSCRANIAL DOPPLER ULTRASOUND [J].
AASLID, R ;
HUBER, P ;
NORNES, H .
JOURNAL OF NEUROSURGERY, 1984, 60 (01) :37-41
[2]   CEREBRAL ARTERIAL SPASM .4. INVITRO EFFECTS OF TEMPERATURE, SEROTONIN ANALOGS, LARGE NONPHYSIOLOGICAL CONCENTRATIONS OF SEROTONIN, AND EXTRACELLULAR CALCIUM AND MAGNESIUM ON SEROTONIN-INDUCED CONTRACTIONS OF CANINE BASILAR ARTERY [J].
ALLEN, GS ;
GROSS, CJ ;
HENDERSON, LM ;
CHOU, SN .
JOURNAL OF NEUROSURGERY, 1976, 44 (05) :585-593
[3]   Impairment of endothelial relaxations by glycosylated human oxyhemoglobin depends on the oxidative state of the heme group [J].
Angulo, J ;
Rodríguez-Mañas, L ;
Peiró, C ;
Vallejo, S ;
Sánchez-Ferrer, A ;
Sanchez-Ferrer, CF .
GENERAL PHARMACOLOGY, 1999, 32 (04) :475-481
[4]   Application of orthogonal signal correction to minimise the effects of physical and biological variation in high resolution 1H NMR spectra of biofluids [J].
Beckwith-Hall, BM ;
Brindle, JT ;
Barton, RH ;
Coen, M ;
Holmes, E ;
Nicholson, JK ;
Antti, H .
ANALYST, 2002, 127 (10) :1283-1288
[5]   Nuclear magnetic resonance spectroscopic and principal components analysis investigations into biochemical effects of three model hepatotoxins [J].
Beckwith-Hall, BM ;
Nicholson, JK ;
Nicholls, AW ;
Foxall, PJD ;
Lindon, JC ;
Connor, SC ;
Abdi, M ;
Connelly, J ;
Holmes, E .
CHEMICAL RESEARCH IN TOXICOLOGY, 1998, 11 (04) :260-272
[6]  
Brindle JT, 2002, NAT MED, V8, P1439, DOI 10.1038/nm802
[7]   HUMAN PRIMARY BRAIN-TUMOR METABOLISM INVIVO - A PHOSPHORUS MAGNETIC-RESONANCE SPECTROSCOPY STUDY [J].
CADOUXHUDSON, TAD ;
BLACKLEDGE, MJ ;
RAJAGOPALAN, B ;
TAYLOR, DJ ;
RADDA, GK .
BRITISH JOURNAL OF CANCER, 1989, 60 (03) :430-436
[8]  
Domingo Z, 2000, NMR BIOMED, V13, P154, DOI 10.1002/1099-1492(200005)13:3<154::AID-NBM620>3.0.CO
[9]  
2-W
[10]   Products of hemolysis in the subarachnoid space inducing spreading ischemia in the cortex and focal necrosis in rats: a model for delayed ischemic neurological deficits after subarachnoid hemorrhage? [J].
Dreier, JP ;
Ebert, N ;
Priller, J ;
Megow, D ;
Lindauer, U ;
Klee, R ;
Reuter, U ;
Imai, Y ;
Einhäupl, KM ;
Victorov, I ;
Dirnagl, U .
JOURNAL OF NEUROSURGERY, 2000, 93 (04) :658-666